| WWOX-mediated apoptosis in A549 cells mainly involves the mitochondrial pathway. | |
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MedLine Citation:
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PMID: 22484428 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The human WWOX gene, known as WW domain-containing oxidoreductase, is located on 16q23.3-24.1, a chromosome region that spans the common fragile site, FRA16D. Abnormal transcripts or even loss of expression are frequently found in a number of cancer cell types, including breast, ovarian, prostate and lung cancer cells. It has therefore been proposed that the WWOX gene encodes a candidate tumor suppressor, possibly a pro-apoptotic protein. However, the mechanism behind this is not entirely clear. In the present study, we examined the pro-apoptotic action of WWOX using transient expression in A549 cells. We observed that the ectopic expression of WWOX caused apoptosis in A549 cells. We further observed procaspase-3 and procaspase-9 activation and the release of cytochrome C from the mitochondria in A549 cells transfected with pcDNA3.0-WWOX. These data indicate that WWOX induces apoptosis in A549 cells via the mitochondrial pathway. |
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Authors:
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Ping Zhang; Renbing Jia; Lei Ying; Bo Liu; Guanxiang Qian; Xianqun Fan; Shengfang Ge |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-04-06 |
Journal Detail:
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Title: Molecular medicine reports Volume: 6 ISSN: 1791-3004 ISO Abbreviation: Mol Med Rep Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-05-09 Completed Date: 2012-09-10 Revised Date: 2013-02-22 |
Medline Journal Info:
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Nlm Unique ID: 101475259 Medline TA: Mol Med Rep Country: Greece |
Other Details:
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Languages: eng Pagination: 121-4 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, Shanghai Jiaotong University School of Medicine, Shanghai, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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genetics,
metabolism Apoptosis* Caspase 3 / metabolism Caspase 9 / metabolism Cell Line, Tumor Cell Proliferation Cytochromes c / metabolism Enzyme Activation / genetics Humans Lung Neoplasms / genetics, metabolism Mitochondria / metabolism* Oxidoreductases / genetics, metabolism* Signal Transduction* Tumor Suppressor Proteins / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Tumor Suppressor Proteins; 9007-43-6/Cytochromes c; EC 1.-/Oxidoreductases; EC 1.1.1.-/WWOX protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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