Document Detail


WWOX-mediated apoptosis in A549 cells mainly involves the mitochondrial pathway.
MedLine Citation:
PMID:  22484428     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The human WWOX gene, known as WW domain-containing oxidoreductase, is located on 16q23.3-24.1, a chromosome region that spans the common fragile site, FRA16D. Abnormal transcripts or even loss of expression are frequently found in a number of cancer cell types, including breast, ovarian, prostate and lung cancer cells. It has therefore been proposed that the WWOX gene encodes a candidate tumor suppressor, possibly a pro-apoptotic protein. However, the mechanism behind this is not entirely clear. In the present study, we examined the pro-apoptotic action of WWOX using transient expression in A549 cells. We observed that the ectopic expression of WWOX caused apoptosis in A549 cells. We further observed procaspase-3 and procaspase-9 activation and the release of cytochrome C from the mitochondria in A549 cells transfected with pcDNA3.0-WWOX. These data indicate that WWOX induces apoptosis in A549 cells via the mitochondrial pathway.
Authors:
Ping Zhang; Renbing Jia; Lei Ying; Bo Liu; Guanxiang Qian; Xianqun Fan; Shengfang Ge
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-04-06
Journal Detail:
Title:  Molecular medicine reports     Volume:  6     ISSN:  1791-3004     ISO Abbreviation:  Mol Med Rep     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-05-09     Completed Date:  2012-09-10     Revised Date:  2013-02-22    
Medline Journal Info:
Nlm Unique ID:  101475259     Medline TA:  Mol Med Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  121-4     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Shanghai Jiaotong University School of Medicine, Shanghai, PR China.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / genetics,  metabolism
Apoptosis*
Caspase 3 / metabolism
Caspase 9 / metabolism
Cell Line, Tumor
Cell Proliferation
Cytochromes c / metabolism
Enzyme Activation / genetics
Humans
Lung Neoplasms / genetics,  metabolism
Mitochondria / metabolism*
Oxidoreductases / genetics,  metabolism*
Signal Transduction*
Tumor Suppressor Proteins / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Tumor Suppressor Proteins; 9007-43-6/Cytochromes c; EC 1.-/Oxidoreductases; EC 1.1.1.-/WWOX protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9

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