Document Detail


The WSX-1 pathway restrains intestinal T-cell immunity.
MedLine Citation:
PMID:  21233255     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mechanisms regulating intestinal T-cell accumulation during inflammation have considerable therapeutic value. In this study, LPS increased Staphylococcus aureus enterotoxin A-specific T cells in the gut through induction of IL-12 family members. Mice deficient in IL-12 (p35(-/-)) favored T(h)17 differentiation in lamina propria, whereas mice lacking both IL-12 and IL-23 (p40(-/-)) produced significantly fewer T(h)17 cells. However, serum analysis revealed that IL-27p28 was much higher and sustained following LPS injection than other IL-12 family cytokines. Strikingly, WSX-1 (IL-27Rα) deficiency resulted in log-fold increases in lamina propria T(h)17 cells without affecting T(h)1 numbers. These results may be explained by increased expression of α4β7 on WSX-1-deficient T cells after immunization. WSX-1-deficient regulatory T cells (Tregs) were also perturbed, producing more IL-17 and less IL-10 than wild-type Tregs. Thus, IL-27 blockade may provide a new pathway to improve mucosal vaccination.
Authors:
Jeremy P McAleer; Christiaan J M Saris; Anthony T Vella
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-01-13
Journal Detail:
Title:  International immunology     Volume:  23     ISSN:  1460-2377     ISO Abbreviation:  Int. Immunol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-01     Completed Date:  2011-05-26     Revised Date:  2012-02-01    
Medline Journal Info:
Nlm Unique ID:  8916182     Medline TA:  Int Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  129-37     Citation Subset:  IM    
Affiliation:
Department of Immunology, University of Connecticut Health Center, Farmington, CT 06030, USA.
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MeSH Terms
Descriptor/Qualifier:
Adjuvants, Immunologic / pharmacology
Animals
Flow Cytometry
Gene Expression Regulation / drug effects
Interleukin-12 / genetics,  immunology*
Interleukin-23 / genetics,  immunology
Intestines / immunology*
Lipopolysaccharides / pharmacology
Mice
Mice, Inbred C57BL
Mice, Knockout
Receptors, Cytokine / genetics,  immunology*
Signal Transduction
T-Lymphocytes / drug effects,  immunology*
Grant Support
ID/Acronym/Agency:
R01-AI42858/AI/NIAID NIH HHS; R01-AI52108/AI/NIAID NIH HHS; T32-AI07080/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Adjuvants, Immunologic; 0/Il27ra protein, mouse; 0/Interleukin-23; 0/Lipopolysaccharides; 0/Receptors, Cytokine; 187348-17-0/Interleukin-12

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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