Document Detail

The WSTF-ISWI chromatin remodeling complex transiently associates with the human inactive X chromosome during late S-phase prior to BRCA1 and γ-H2AX.
MedLine Citation:
PMID:  23166813     Owner:  NLM     Status:  MEDLINE    
Replicating the genome prior to each somatic cell division not only requires precise duplication of the genetic information, but also accurately reestablishing the epigenetic signatures that instruct how the genetic material is to be interpreted in the daughter cells. The mammalian inactive X chromosome (Xi), which is faithfully inherited in a silent state in each daughter cell, provides an excellent model of epigenetic regulation. While much is known about the early stages of X chromosome inactivation, much less is understood with regards to retaining the Xi chromatin through somatic cell division. Here we report that the WSTF-ISWI chromatin remodeling complex (WICH) associates with the Xi during late S-phase as the Xi DNA is replicated. Elevated levels of WICH at the Xi is restricted to late S-phase and appears before BRCA1 and γ-H2A.X. The sequential appearance of WICH and BRCA1/γ-H2A.X implicate each as performing important but distinct roles in the maturation and maintenance of heterochromatin at the Xi.
Ashley E Culver-Cochran; Brian P Chadwick
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-11-14
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-11-20     Completed Date:  2013-04-29     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e50023     Citation Subset:  IM    
Department of Biological Science, Florida State University, Tallahassee, Florida, United States of America.
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MeSH Terms
Adenosine Triphosphatases / metabolism
BRCA1 Protein / metabolism
Chromatin Assembly and Disassembly / genetics,  physiology*
Chromosomes, Human, X / genetics,  metabolism*
DNA Replication / physiology*
Epigenesis, Genetic / genetics,  physiology*
Fluorescent Antibody Technique
Histones / metabolism
Image Processing, Computer-Assisted
In Situ Hybridization, Fluorescence
S Phase / physiology*
Transcription Factors / metabolism
X Chromosome Inactivation / genetics*
Grant Support
Reg. No./Substance:
0/BAZ1B protein, human; 0/BRCA1 Protein; 0/H2AFX protein, human; 0/Histones; 0/ISWI protein; 0/Transcription Factors; 59-14-3/Bromodeoxyuridine; EC 3.6.1.-/Adenosine Triphosphatases

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