| WNT3A modulates chondrogenesis via canonical and non-canonical Wnt pathways in MSCs . | |
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MedLine Citation:
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PMID: 23276938 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The multilineage commitment of mesenchymal stem cells (MSCs) is controlled via unknown mechanisms. In this study, we investigated the regulation of the differentiation of MSCs into chondrocytes via the Wnt signaling pathway. Overexpression of WNT3A in MSCs activated both the canonical and non-canonical Wnt pathways, which were responsible for different WNT3A-induced outcomes. WNT3A promoted MSC proliferation via the ß-catenin-mediated canonical Wnt pathway, and inhibited chondrogenesis of MSCs via the calcium/calmodulin-dependent kinase II (CaMKII)-mediated non-canonical Wnt pathway. Interestingly, blockade of the canonical Wnt pathway by Dickkopf-related protein 1 exerted a synergistic effect on the inhibition of chondrogenesis of MSCs, while blockade of the non-canonical Wnt pathway by KN93 also exerted a synergistic effect on MSCs proliferation. These results suggest that the WNT3A-activated canonical and non-canonical pathways counteract each other in the setting of MSCs. This study provides evidence for the delicate regulation of the Wnt signaling cascade during chondrogenesis of MSCs, and suggests that genetic manipulation of the Wnt pathway may offer a powerful vehicle for modulating MSC differentiation in stem-cell-based cartilage repair. |
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Authors:
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Feng Qu; Junliang Wang; Ningru Xu; Chang Liu; Shuyuan Li; Ning Wang; Wei Qi; Haifeng Li; Chunbao Li; Zhenying Geng; Yujie Liu |
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Publication Detail:
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Type: Journal Article Date: 2013-01-01 |
Journal Detail:
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Title: Frontiers in bioscience : a journal and virtual library Volume: 18 ISSN: 1093-4715 ISO Abbreviation: Front. Biosci. Publication Date: 2013 |
Date Detail:
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Created Date: 2013-01-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9709506 Medline TA: Front Biosci Country: United States |
Other Details:
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Languages: eng Pagination: 493-503 Citation Subset: IM |
Affiliation:
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Department of orthopedics, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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