Document Detail


A WFS1 haplotype consisting of the minor alleles of rs752854, rs10010131, and rs734312 shows a protective role against type 2 diabetes in Russian patients.
MedLine Citation:
PMID:  21713316     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Rare variants of the WFS1 gene encoding wolframin cause Wolfram syndrome, a monogenic disease associated with diabetes insipidus, diabetes mellitus, optic atrophy, and deafness. In contrast, common variants of WFS1 showed association with type 2 diabetes (T2D) in numerous Caucasian populations.
AIM: In this study, we tested whether the markers rs752854, rs10010131, and rs734312, located in the WFS1 gene, are related to the development of T2D in a Russian population.
METHODS: The polymorphic markers were genotyped in Russian diabetic (n = 1,112) and non-diabetic (n = 1,097) patients using a Taqman allele discrimination assay. The correlation between the carriage of disease-associated WFS1 variants and the patients' clinical and metabolic characteristics was studied using ANOVA and ANCOVA. Adjustment for confounding variables such as gender, age, body mass index, obesity, HbA1c, and hypertension was made.
RESULTS: Haplotype GAG, consisting of the minor alleles of rs752854, rs10010131, and rs734312, respectively, showed association with decreased risk of T2D (OR = 0.44, 95% CI = 0.32-0.61, p = 4.3 x 10(-7)). Compared to other WFS1 variants, non-diabetic individuals homozygous for GAG/CAG had significantly increased fasting insulin (p(adjusted) = 0.047) and homeostasis model assessment of β-cell function (HOMA-β) index (p(adjusted) = 0.006). Diabetic patients homozygous for GAG/GAG showed significantly elevated levels of 2-h insulin (p(adjusted) = 0.029) and HOMA-β = 0.011.
CONCLUSIONS: Disease-associated variants of WFS1 contribute to the pathogenesis of T2D through impaired insulin response to glucose stimulation and altered β-cell function.
Authors:
Dimitry A Chistiakov; Dmitry S Khodyrev; Svetlana A Smetanina; Larisa N Bel'chikova; Lyudmila A Suplotova; Valery V Nosikov
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-02-10
Journal Detail:
Title:  The review of diabetic studies : RDS     Volume:  7     ISSN:  1614-0575     ISO Abbreviation:  Rev Diabet Stud     Publication Date:  2010  
Date Detail:
Created Date:  2011-06-29     Completed Date:  2011-10-27     Revised Date:  2012-02-10    
Medline Journal Info:
Nlm Unique ID:  101227575     Medline TA:  Rev Diabet Stud     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  285-92     Citation Subset:  IM    
Affiliation:
National Research Center GosNIIgenetika, Moscow, Russia. dimitry.chistiakov@lycos.com
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MeSH Terms
Descriptor/Qualifier:
Aged
Alleles*
Base Sequence
Diabetes Mellitus, Type 2 / genetics*
Female
Haplotypes*
Humans
Male
Membrane Proteins / genetics*
Middle Aged
Molecular Sequence Data
Russia
Chemical
Reg. No./Substance:
0/Membrane Proteins; 0/wolframin protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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