| A WD-repeat protein stabilizes ORC binding to chromatin. | |
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MedLine Citation:
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PMID: 20932478 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Origin recognition complex (ORC) plays critical roles in the initiation of DNA replication and cell-cycle progression. In metazoans, ORC associates with origin DNA during G1 and with heterochromatin in postreplicated cells. However, what regulates the binding of ORC to chromatin is not understood. We have identified a highly conserved, leucine-rich repeats and WD40 repeat domain-containing protein 1 (LRWD1) or ORC-associated (ORCA) in human cells that interacts with ORC and modulates chromatin association of ORC. ORCA colocalizes with ORC and shows similar cell-cycle dynamics. We demonstrate that ORCA efficiently recruits ORC to chromatin. Depletion of ORCA in human primary cells and embryonic stem cells results in loss of ORC association to chromatin, concomitant reduction of MCM binding, and a subsequent accumulation in G1 phase. Our results suggest ORCA-mediated association of ORC to chromatin is critical to initiate preRC assembly in G1 and chromatin organization in post-G1 cells. |
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Authors:
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Zhen Shen; Kizhakke M Sathyan; Yijie Geng; Ruiping Zheng; Arindam Chakraborty; Brian Freeman; Fei Wang; Kannanganattu V Prasanth; Supriya G Prasanth |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Molecular cell Volume: 40 ISSN: 1097-4164 ISO Abbreviation: Mol. Cell Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-11 Completed Date: 2010-11-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9802571 Medline TA: Mol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 99-111 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, 601S Goodwin Avenue, Urbana, IL 61801, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptor Proteins, Signal Transducing
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genetics,
metabolism* Amino Acid Sequence Animals Binding Sites Cell Cycle Cell Line, Tumor Cell Proliferation Chromatin Assembly and Disassembly* Conserved Sequence Fluorescent Antibody Technique Heterochromatin / metabolism* Humans Immunoprecipitation Microscopy, Video Molecular Sequence Data Mutation Origin Recognition Complex / genetics, metabolism* Protein Binding Protein Interaction Domains and Motifs RNA Interference Recombinant Fusion Proteins / metabolism Time Factors Transfection |
| Chemical | |
Reg. No./Substance:
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0/Adaptor Proteins, Signal Transducing; 0/Heterochromatin; 0/Origin Recognition Complex; 0/Recombinant Fusion Proteins; 0/SQSTM1 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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