Document Detail


A σW-dependent stress response in Bacillus subtilis that reduces membrane fluidity.
MedLine Citation:
PMID:  21542858     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacteria respond to physical and chemical stresses that affect the integrity of the cell wall and membrane by activating an intricate cell envelope stress response. The ability of cells to regulate the biophysical properties of the membrane by adjusting fatty acid composition is known as homeoviscous adaptation. Here, we identify a homeoviscous adaptation mechanism in Bacillus subtilis regulated by the extracytoplasmic function σ factor σ(W). Cell envelope active compounds, including detergents, activate a sense-oriented, σ(W)-dependent promoter within the first gene of the fabHa fabF operon. Activation leads to a decrease in the amount of FabHa coupled with an increase in FabF, the initiation and elongation condensing enzymes of fatty acid biosynthesis respectively. Downregulation of FabHa results in an increased reliance on the FabHb paralogue leading to a greater proportion of straight chain fatty acids in the membrane, and the upregulation of FabF increases the average fatty acid chain length. The net effect is to reduce membrane fluidity. The inactivation of the σ(W)-dependent promoter within fabHa increased sensitivity to detergents and to antimicrobial compounds produced by other Bacillus spp. Thus, the σ(W) stress response provides a mechanism to conditionally decrease membrane fluidity through the opposed regulation of FabHa and FabF.
Authors:
Anthony W Kingston; Chitra Subramanian; Charles O Rock; John D Helmann
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-06-09
Journal Detail:
Title:  Molecular microbiology     Volume:  81     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-27     Completed Date:  2011-10-13     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  69-79     Citation Subset:  IM    
Copyright Information:
© 2011 Blackwell Publishing Ltd.
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / toxicity
Bacillus subtilis / drug effects,  physiology*
Bacterial Proteins / metabolism*
Cell Membrane / metabolism
Detergents / toxicity
Fatty Acids / metabolism
Membrane Fluidity*
Operon
Promoter Regions, Genetic
Sigma Factor / metabolism*
Stress, Physiological*
Grant Support
ID/Acronym/Agency:
CA21765/CA/NCI NIH HHS; GM034496/GM/NIGMS NIH HHS; GM47446/GM/NIGMS NIH HHS; R01 GM034496/GM/NIGMS NIH HHS; R01 GM047446/GM/NIGMS NIH HHS; R01 GM047446-19/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/Detergents; 0/Fatty Acids; 0/SigW protein, Bacillus subtilis; 0/Sigma Factor
Comments/Corrections

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