| The W- and Z-linked EE0.6 sequences used for molecular sexing of captive Japanese crested ibis on Sado Island. | |
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MedLine Citation:
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PMID: 22250744 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The Japanese crested ibis Nipponia nippon is a critically threatened bird. Accurate sexing is necessary to perform effective management of captive breeding toward a national project for a tentative release of the Japanese crested ibis on Sado Island. A PCR-based sexing method targeting a 0.6 kb EcoRI fragment (EE0.6) sequence on W chromosome with AWS03 and USP3 primers has been developed for the Japanese crested ibis. However, the primers were selected from the EE0.6 sequences from bird species other than the Japanese crested ibis. In this study, we determined the W- and Z-linked EE0.6 sequences in the Japanese crested ibis, and clarified Japanese crested ibis sequence mismatch in the binding sites of the primers. Further, we found no polymorphism in the primer binding sites among five founder birds for the Sado captive Japanese crested ibis population. These findings validated the PCR-based sexing method with the AWS03 and USP3 as accurate molecular sexing methods of captive Japanese crested ibis on the Sado Island. Additionally, we designed a primer set for a novel PCR-based sexing, based on the EE0.6 sequences obtained in this study. This novel sexing method may be useful for future ecological research following the release of Japanese crested ibis on Sado Island. This is the first report to show the EE0.6 sequences in Japanese crested ibis. |
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Authors:
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Kaori Kasuga; Maho Higashi; Takahisa Yamada; Toshie Sugiyama; Yukio Taniguchi; Hiroaki Iwaisaki; Kosuke Homma; Yuuichi Wajiki; Yoshinori Kaneko; Satoshi Yamagishi |
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Publication Detail:
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Type: Journal Article Date: 2011-10-18 |
Journal Detail:
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Title: Animal science journal = Nihon chikusan Gakkaihō Volume: 83 ISSN: 1740-0929 ISO Abbreviation: Anim. Sci. J. Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-01-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100956805 Medline TA: Anim Sci J Country: Australia |
Other Details:
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Languages: eng Pagination: 83-7 Citation Subset: IM |
Copyright Information:
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© 2011 The Authors. Animal Science Journal © 2011 Japanese Society of Animal Science. |
Affiliation:
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Department of Agrobiology, Faculty of Agriculture, Niigata University, Nishi, Niigata Laboratory of Animal Breeding and Genetics, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto Field Center for Sustainable Agriculture and Forestry, Niigata University, Nishi, Niigata Sado Japanese crested ibis Conservation Center, Sado, Niigata Yamashina Institute for Ornithology, Abiko, Chiba, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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