Document Detail


Vps1 in the late endosome-to-vacuole traffic.
MedLine Citation:
PMID:  23385815     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Vacuolar protein sorting 1 (Vps1), the yeast homolog to human dynamin, is a GTP hydrolyzing protein, which plays an important role in protein sorting and targeting between the Golgi and late endosomal compartments. In this study, we assessed the functional significance of Vps1 in the membrane traffic towards the vacuole. We show here that vps1 delta cells accumulated FM4-64 to a greater extent than wild-type (WT))cells, suggesting slower endocytic degradation traffic toward the vacuole. In addition, we observed that two endosome-to-vacuole traffic markers, DsRed-FYVE and Ste2-GFP, were highly accumulated in Vps1-deficient cells, further supporting Vps1's implication in efficient trafficking of endocytosed materials to the vacuole. Noteworthy, a simultaneous imaging analysis in conjunction with FM4-64 pulse-chase experiment further revealed that Vps1 plays a role in late endosome to the vacuole transport. Consistently, our subcellular localization analysis showed that Vps1 is present at the late endosome. The hyperaccumulation of endosomal intermediates in the vps1 mutant cells appears to be caused by the disruption of integrity of HOPS tethering complexes, manifested by mislocalization of Vps39 to the cytoplasm. Finally, we postulate that Vps1 functions together with the Endosomal Sorting Complex Required for Transport (ESCRT) complex at the late endosomal compartments, based on the observation that the double mutants, in which VPS1 along with singular ESCRT I, II and III genes have been disrupted, exhibited synthetic lethality. Together, we propose that Vps1 is required for correct and efficient trafficking from the late endosomal compartments to the vacuole.
Authors:
Jacob Hayden; Michelle Williams; Ann Granich; Hyoeun Ahn; Brandon Tenay; Joshua Lukehart; Chad Highfill; Sarah Dobard; Kyoungtae Kim
Related Documents :
23251325 - Depending on its nano-spacing, alcam promotes cell attachment and axon growth.
23765165 - Transport through the golgi in trypanosoma brucei.
24586495 - Cystic fibrosis bronchial epithelial cells are lipointoxicated by membrane palmitate ac...
23413785 - Differential interactions of streptococcus gordonii and staphylococcus aureus with cult...
2905975 - Acetylcholinesterase and butyrylcholinesterase in the gut mucosal cells of various mamm...
15599055 - Unusual distribution of mitochondrial large subunit rrna in the cytosol during conjugat...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biosciences     Volume:  38     ISSN:  0973-7138     ISO Abbreviation:  J. Biosci.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100809     Medline TA:  J Biosci     Country:  India    
Other Details:
Languages:  eng     Pagination:  73-83     Citation Subset:  IM    
Affiliation:
Department of Biology, Missouri State University, 901 S National, Springfield, Missouri 65807.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Fatty acyl chain-dependent but charge-independent association of the SH4 domain of Lck with lipid me...
Next Document:  Cellular ontogeny of RBMY during human spermatogenesis and its role in sperm motility.