Document Detail

Voluntary wheel running is beneficial to the amino acid profile of lysine-deficient rats.
MedLine Citation:
PMID:  20233939     Owner:  NLM     Status:  MEDLINE    
Rats voluntarily run up to a dozen kilometers per night when their cages are equipped with a running wheel. Daily voluntary running is generally thought to enhance protein turnover. Thus, we sought to determine whether running worsens or improves protein degradation caused by a lysine-deficient diet and whether it changes the utilization of free amino acids released by proteolysis. Rats were fed a lysine-deficient diet and were given free access to a running wheel or remained sedentary (control) for 4 wk. Amino acid levels in plasma, muscle, and liver were measured together with plasma insulin levels and tissue weight. The lysine-deficient diet induced anorexia, skeletal muscle loss, and serine and threonine aminoacidemia, and it depleted plasma insulin and essential amino acids in skeletal muscle. Allowing rats to run voluntarily improved these symptoms; thus, voluntary wheel running made the rats less susceptible to dietary lysine deficiency. Amelioration of the declines in muscular leucine and plasma insulin observed in running rats could contribute to protein synthesis together with the enhanced availability of lysine and other essential amino acids in skeletal muscle. These results indicate that voluntary wheel running under lysine-deficient conditions does not enhance protein catabolism; on the contrary, it accelerates protein synthesis and contributes to the maintenance of muscle mass. The intense nocturnal voluntary running that characterizes rodents might be an adaptation of lysine-deficient grain eaters that allows them to maximize opportunities for food acquisition.
Kenji Nagao; Makoto Bannai; Shinobu Seki; Nobuhiro Kawai; Masato Mori; Michio Takahashi
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Publication Detail:
Type:  Journal Article     Date:  2010-03-16
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  298     ISSN:  1522-1555     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-14     Completed Date:  2010-06-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E1170-8     Citation Subset:  IM    
Institute of Life Sciences, Ajinomoto Co. Inc., Kawasaki-ku, Japan.
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MeSH Terms
Amino Acids / metabolism*
Argininosuccinate Lyase / genetics,  metabolism
Insulin / blood
Liver / enzymology,  metabolism
Lysine / deficiency*,  metabolism
Motor Activity / physiology*
Muscle Proteins / biosynthesis,  metabolism
Muscle, Skeletal / metabolism*
Phosphoglycerate Dehydrogenase / genetics,  metabolism
RNA / chemistry,  genetics
Random Allocation
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Urea / blood
Reg. No./Substance:
0/Amino Acids; 0/Muscle Proteins; 11061-68-0/Insulin; 56-87-1/Lysine; 57-13-6/Urea; 63231-63-0/RNA; EC Dehydrogenase; EC Lyase

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