Document Detail

Volumetric assessment and quantitative element analysis of the effect of fluoridated milk on enamel demineralization.
MedLine Citation:
PMID:  12749919     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: It was the purpose of this study to investigate the effect of milk and fluoridated milk on experimentally induced caries-like-lesions. MATERIALS AND METHODS: Twelve extracted impacted human molars were covered with wax leaving two 3 mm x 3 mm windows on the buccal and lingual surfaces and incubated alternating in demineralizing solution at pH 4.85 and in milk, F-milk, saline, and remineralizing solution, respectively. Afterwards serial ground sections were cut and investigated by polarization-light-microscopy (PLM) and SEM using EDX element analysis. RESULTS: The results showed increased thickness of the superficial layer in the F-milk samples. Quantitative element analysis revealed a significant increase in the fluoride content of the superficial layer and of the body of the lesion of the F-milk group. The body of the lesion of the F-milk group was less demineralized than in the other groups. CONCLUSION: From the results we conclude, that the parallel investigation by PLM, SEM combined with EDX quantitative element analysis is a powerful method to assess caries-like-lesion formation and that fluoridated milk has protective properties in inhibiting demineralization.
W H Arnold; M Cerman; K Neuhaus; P Gaengler
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of oral biology     Volume:  48     ISSN:  0003-9969     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-05-16     Completed Date:  2003-07-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  467-73     Citation Subset:  D; IM    
Department of Anatomy, Faculty of Dental Medicine, University of Witten/Herdecke, Alfred Herrhausen Strasse 50, 58448, Witten, Germany. <>
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MeSH Terms
Dental Caries / metabolism,  pathology,  prevention & control*
Dental Enamel / chemistry,  drug effects*,  ultrastructure
Fluorides / analysis
Microscopy, Electron, Scanning
Microscopy, Polarization
X-Ray Diffraction
Reg. No./Substance:

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