Document Detail


Volume-sensitive Ca influx and release from intracellular pools in gastric parietal cells.
MedLine Citation:
PMID:  1415509     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of osmotically induced changes in cell volume on cytoplasmic free Ca (Cai) were studied in parietal cells from intact rabbit gastric glands using digital image processing of fura-2 fluorescence. In resting unstimulated cells, Cai was unaffected by either cell swelling or shrinking when osmolarity was varied between 200 and 400 mosM (isotonicity 290 mosM). However, when cells were swelled in a 165 mosM solution (55% tonicity), a biphasic Ca increased was observed. On average, Cai increased transiently from 80 to 218 nM before stabilizing at approximately 140 nM. The peak was due to release from intracellular pools because it was present in Ca-free solutions while the sustained elevation was dependent on external Ca. In carbachol-stimulated cells, Ca influx was most sensitive to cell shrinkage. For example, addition of 25 mM sucrose (108% tonicity) caused a 30% decrease in the sustained carbachol-stimulated Cai increase (plateau). In contrast, carbachol-stimulated cells were relatively insensitive to cell swelling, with a 30% decrease in tonicity causing only a 15% increase in the plateau. However, as in the unstimulated cells, extreme (55% tonicity) swelling caused additional increases in Cai levels. The carbachol-dependent effects of changes in cell volume on Cai could be mimicked by treating cells with thapsigargin, an inhibitor of Ca pumps of intracellular membranes that also has been shown to stimulate Ca entry. Thus, although extreme swelling conditions (55% tonicity) could elicit Cai increases in either the presence or absence of agonist, agonist was required to observe Cai decreases due to cell shrinkage.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
P A Negulescu; B Munck; T E Machen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  263     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1992 Sep 
Date Detail:
Created Date:  1992-10-29     Completed Date:  1992-10-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C584-9     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / metabolism*
Calcium-Transporting ATPases / antagonists & inhibitors
Carbachol / pharmacology
Hypertonic Solutions / pharmacology
Hypotonic Solutions / pharmacology
Intracellular Membranes / metabolism*
Parietal Cells, Gastric / metabolism*
Terpenes / pharmacology
Thapsigargin
Grant Support
ID/Acronym/Agency:
DK-08238/DK/NIDDK NIH HHS; DK-19520/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Hypertonic Solutions; 0/Hypotonic Solutions; 0/Terpenes; 51-83-2/Carbachol; 67526-95-8/Thapsigargin; 7440-70-2/Calcium; EC 3.6.1.8/Calcium-Transporting ATPases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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