Document Detail

Volume regulation in the human airway epithelial cell line Calu-3.
MedLine Citation:
PMID:  19448731     Owner:  NLM     Status:  MEDLINE    
Cells regulate their volume in response to changes in the osmolarity of both their extracellular and their intracellular environments. We investigated the ability of the human airway epithelial cell line Calu-3 to respond to changes in extracellular osmolarity. Although switching Calu-3 cells from an isosmotic to a hyperosmotic environment resulted in cell shrinkage, there was no compensatory mechanism for the cells to return to their original volume. In contrast, switching to a hyposmotic environment resulted in an initial cell swelling response, followed by a regulatory volume decrease (RVD). Pharmacologic studies demonstrate that the voltage-activated K+ channels Kv4.1 and (or) Kv4.3 play a crucial role in mediating this RVD response, and we demonstrated expression of these channel types at the mRNA and protein levels. Furthermore, inhibition of the large- and intermediate-conductance Ca2+-activated K+ channels KCa1.1 (maxi-K) and KCa3.1 (hIK) also implicated these channels as playing a role in volume recovery in Calu-3 cells. This report describes the nature of volume regulation in the widely used model cell line Calu-3.
Scott A Harron; Christina M Clarke; Christina L Jones; Dominique Babin-Muise; Elizabeth A Cowley
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of physiology and pharmacology     Volume:  87     ISSN:  0008-4212     ISO Abbreviation:  Can. J. Physiol. Pharmacol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-18     Completed Date:  2009-08-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372712     Medline TA:  Can J Physiol Pharmacol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  337-46     Citation Subset:  IM    
Department of Physiology and Biophysics, Dalhousie University, 5850 College Street, Halifax, NS B3H 1X5, Canada.
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MeSH Terms
Bronchi / cytology*,  physiology
Cell Line
Cell Size*
Chloride Channels / physiology
Cystic Fibrosis Transmembrane Conductance Regulator / physiology
Epithelial Cells / cytology*
KCNQ1 Potassium Channel / physiology
Osmotic Pressure
Potassium Channels / physiology
Trachea / cytology*,  physiology
Reg. No./Substance:
0/Chloride Channels; 0/KCNQ1 Potassium Channel; 0/KCNQ1 protein, human; 0/Potassium Channels; 126880-72-6/Cystic Fibrosis Transmembrane Conductance Regulator

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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