Document Detail

Volume changes of mature human oocytes on exposure to cryoprotectant solutions used in slow cooling procedures.
MedLine Citation:
PMID:  15665009     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Despite the recent increase in pregnancies from cryopreserved human oocytes, success in terms of births per thawed oocyte is still poor. Modifications to cryopreservation protocols have not been based on measurement of the osmotic response of oocytes, and methodologies are often poorly described or protocols not strictly adhered to, inevitably resulting in variability. METHODS: Volume change of mature human oocytes was measured on exposure to cryoprotectant. Oocytes were exposed to either 0.75 mol/l propane-1,2-diol (PrOH) for 10 min; 1.5 mol/l PrOH for 10 min, having been exposed to 0.75 mol/l PrOH for 7.5 min; or 1.5 mol/l PrOH plus 0.2 or 0.3 mol/l sucrose for 10 min, having been exposed to 1.5 mol/l PrOH for 10 min. RESULTS: On exposure to PrOH alone, oocytes shrank and then re-expanded, having reached 75 and 84% of their starting volume in 0.75 and 1.5 mol/l, respectively. Oocytes shrank continuously in PrOH plus sucrose, reaching 67 or 55% of their initial volume in 0.2 or 0.3 mol/l sucrose, respectively. CONCLUSIONS: To improve consistency following cryopreservation, protocols must be strictly adhered to; small changes in duration of exposure to cryoprotectant can result in drastic changes in cellular hydration and thus the fate of the cell during freezing/thawing.
S J Paynter; A Borini; V Bianchi; L De Santis; C Flamigni; G Coticchio
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Publication Detail:
Type:  Journal Article     Date:  2005-01-21
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  20     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-04-22     Completed Date:  2005-08-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  1194-9     Citation Subset:  IM    
Department of Obstetrics and Gynaecology, Wales College of Medicine, Cardiff University, UK.
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MeSH Terms
Cell Size / drug effects
Cryopreservation / methods*
Cryoprotective Agents / pharmacology*
Oocytes / cytology*,  drug effects*
Osmotic Pressure
Propylene Glycol / pharmacology
Sucrose / pharmacology
Reg. No./Substance:
0/Cryoprotective Agents; 0/Solutions; 57-50-1/Sucrose; 57-55-6/Propylene Glycol

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