Document Detail


Volume-activated taurine transport is differentially activated in human cervical cancer HT-3 cells but not in human papillomavirus-immortalized Z183A and normal cervical epithelial cells.
MedLine Citation:
PMID:  9406659     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. Previous studies demonstrate that volume-sensitive chloride currents are distinctly activated in cervical cancer cells, but not in human papillomavirus (HPV)-immortalized and normal cervical cells. In the present study, the Na(+)-independent volume-activated transport of taurine in three cervical cell types was investigated. 2. Osmotic swelling of cervical cancer HT-3 cells suspended in Na(+)-free hypotonic medium led to increased membrane uptake of taurine. This taurine uptake was effectively blocked by various Cl- channel blockers with a similar potency in blocking volume-sensitive Cl- channels: 1,9-dideoxyforskolin > 5-nitro-2-(3-phenyl-propylamino)-benzoic acid (NPPB) > 4-acetamido-4'-isothiocyanastilbene-2,2'-disulphonic acid (SITS) > 4,4'-diisothio-cyanatostilbene-2,2-disulphonic acid (DIDS) > furosemide. The taurine influx was also abolished by pertussis toxin. In contrast, Na(+)-independent volume-activated taurine transport was not significantly activated in HPV-immortalized Z183A cells and in normal cervical cells. 3. Exposure of HT-3 cells to hypotonic medium also resulted in a marked increase in taurine efflux. The volume-activated taurine efflux was osmolarity dependent and the pattern of pharmacological inhibition by Cl- channel blockers was indistinguishable from that for taurine uptake. 4. These results suggest that volume-sensitive Cl- channels in HT-3 cells can mediate the transport of amino acids. In addition, the pertussis toxin-sensitive G-protein is linked with the activation of this transport mechanism.
Authors:
C Y Chou; M R Shen; T M Chen; K E Huang
Related Documents :
12177159 - Mechanisms of acid secretion in pseudobranch cells of rainbow trout (oncorhynchus mykiss).
3811979 - Carbonic anhydrase inhibition and cell volume regulation in necturus gallbladder.
8647909 - Protein patterns, osmolytes, and aldose reductase of l-929 cells exposed to hyperosmoti...
16520829 - A role for ion channels in glioma cell invasion.
3595179 - Sodium and bicarbonate fluxes in regenerating rabbit corneal endothelium.
15922329 - Snat2 silencing prevents the osmotic induction of transport system a and hinders cell r...
4886279 - Properties of a cell fraction that repairs damage to the cell division mechanism of esc...
1798289 - Requirement for prooxidant and antioxidant states in t cell mediated immune responses.-...
3148609 - Quantitative evaluation of bacteria washed from stainless steel penicylinders during ao...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental pharmacology & physiology     Volume:  24     ISSN:  0305-1870     ISO Abbreviation:  Clin. Exp. Pharmacol. Physiol.     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-01-12     Completed Date:  1998-01-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0425076     Medline TA:  Clin Exp Pharmacol Physiol     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  935-9     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, National Cheng Kung University Medical College, Tainan, Taiwan. chougyn@mail.ncku.edu.tw
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biological Transport, Active / drug effects
Cell Size / physiology
Cell Transformation, Viral*
Cervix Uteri / cytology,  metabolism*,  virology*
Chloride Channels / antagonists & inhibitors,  metabolism
Epithelial Cells / metabolism
Female
Humans
Hypotonic Solutions
Osmotic Pressure
Papillomaviridae*
Taurine / pharmacokinetics*
Tritium
Tumor Cells, Cultured
Uterine Cervical Neoplasms / metabolism*,  pathology
Chemical
Reg. No./Substance:
0/Chloride Channels; 0/Hypotonic Solutions; 10028-17-8/Tritium; 107-35-7/Taurine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Potentiation of dipsogenic actions by centrally administered type-C natriuretic peptide in spontaneo...
Next Document:  Autonomic control of bronchial circulation in awake sheep during rest and behaviour.