Document Detail

Volume-activated taurine transport is differentially activated in human cervical cancer HT-3 cells but not in human papillomavirus-immortalized Z183A and normal cervical epithelial cells.
MedLine Citation:
PMID:  9406659     Owner:  NLM     Status:  MEDLINE    
1. Previous studies demonstrate that volume-sensitive chloride currents are distinctly activated in cervical cancer cells, but not in human papillomavirus (HPV)-immortalized and normal cervical cells. In the present study, the Na(+)-independent volume-activated transport of taurine in three cervical cell types was investigated. 2. Osmotic swelling of cervical cancer HT-3 cells suspended in Na(+)-free hypotonic medium led to increased membrane uptake of taurine. This taurine uptake was effectively blocked by various Cl- channel blockers with a similar potency in blocking volume-sensitive Cl- channels: 1,9-dideoxyforskolin > 5-nitro-2-(3-phenyl-propylamino)-benzoic acid (NPPB) > 4-acetamido-4'-isothiocyanastilbene-2,2'-disulphonic acid (SITS) > 4,4'-diisothio-cyanatostilbene-2,2-disulphonic acid (DIDS) > furosemide. The taurine influx was also abolished by pertussis toxin. In contrast, Na(+)-independent volume-activated taurine transport was not significantly activated in HPV-immortalized Z183A cells and in normal cervical cells. 3. Exposure of HT-3 cells to hypotonic medium also resulted in a marked increase in taurine efflux. The volume-activated taurine efflux was osmolarity dependent and the pattern of pharmacological inhibition by Cl- channel blockers was indistinguishable from that for taurine uptake. 4. These results suggest that volume-sensitive Cl- channels in HT-3 cells can mediate the transport of amino acids. In addition, the pertussis toxin-sensitive G-protein is linked with the activation of this transport mechanism.
C Y Chou; M R Shen; T M Chen; K E Huang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental pharmacology & physiology     Volume:  24     ISSN:  0305-1870     ISO Abbreviation:  Clin. Exp. Pharmacol. Physiol.     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-01-12     Completed Date:  1998-01-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0425076     Medline TA:  Clin Exp Pharmacol Physiol     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  935-9     Citation Subset:  IM    
Department of Obstetrics and Gynecology, National Cheng Kung University Medical College, Tainan, Taiwan.
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MeSH Terms
Biological Transport, Active / drug effects
Cell Size / physiology
Cell Transformation, Viral*
Cervix Uteri / cytology,  metabolism*,  virology*
Chloride Channels / antagonists & inhibitors,  metabolism
Epithelial Cells / metabolism
Hypotonic Solutions
Osmotic Pressure
Taurine / pharmacokinetics*
Tumor Cells, Cultured
Uterine Cervical Neoplasms / metabolism*,  pathology
Reg. No./Substance:
0/Chloride Channels; 0/Hypotonic Solutions; 10028-17-8/Tritium; 107-35-7/Taurine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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