Document Detail

Volume-activated chloride channels contribute to cell-cycle-dependent regulation of HeLa cell migration.
MedLine Citation:
PMID:  18992227     Owner:  NLM     Status:  MEDLINE    
The activation of volume-activated chloride Cl(-) channels has been implicated to play important roles in modulating cell cycle and cell migration. The aim of this study was to determine whether volume-activated Cl(-) channels are involved in cell-cycle-dependent regulation of cell migration in HeLa cells. Using techniques including cell-cycle synchronization, transwell migration assays and the patch-clamp technique, we demonstrate in this study that both the expression of volume-activated chloride current (I(Cl,vol)) and the potential of cell migration are cell-cycle-dependent; specifically, these events were high in G(0)/G(1) phase, low in S phase, and medium in G(2)/M phase. Moreover, the mean density of I(Cl,vol) was positively correlated to the rate of cell migration during cell-cycle progression. Additionally, endogenous suppression of I(Cl,vol) by transfecting cells with ClC-3 antisense oligonucleotides arrested cells in S phase and slowed cell migration. Collectively, our results suggest that volume-activated Cl(-) channels contribute to the cell-cycle-dependent regulation of cell migration.
Jianwen Mao; Lixin Chen; Bin Xu; Lijing Wang; Weizhang Wang; Ming Li; Min Zheng; Hongzhi Li; Jiao Guo; Weidong Li; Tim J C Jacob; Liwei Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-15
Journal Detail:
Title:  Biochemical pharmacology     Volume:  77     ISSN:  1873-2968     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-16     Completed Date:  2009-02-03     Revised Date:  2009-05-21    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  159-68     Citation Subset:  IM    
Institute of Basic Medical Sciences and Department of Biology, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangdong, China.
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MeSH Terms
Cell Cycle / physiology*
Cell Division
Cell Membrane / drug effects,  physiology
Cell Movement
Chloride Channels / drug effects,  physiology*
Demecolcine / pharmacology
Electrophysiology / methods
G2 Phase
Hela Cells / cytology,  drug effects,  physiology*
Hydroxyurea / pharmacology
Patch-Clamp Techniques
S Phase
Uterine Cervical Neoplasms
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Chloride Channels; 127-07-1/Hydroxyurea; 477-30-5/Demecolcine

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