Document Detail


Voltage-dependent calcium and chloride currents in S17 bone marrow stromal cell line.
MedLine Citation:
PMID:  20049895     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The bone marrow stromal cell line S17 has been used to study hematopoiesis in vitro. In this study, we demonstrate the presence of calcium and chloride currents in cultured S17 cells. Calcium currents were of low amplitude or barely detectable (50-100 pA). Hence to amplify the currents, we have used barium as a charge carrier. Barium currents were identified based on their distinct voltage-dependence, and sensitivity to dihydropyridines. S17 cells also exhibited a slowly activating outward current without inactivation, most commonly seen when the sodium of the extracellular solution was replaced either by TEA (TEA/Cs saline) or NMDG (NMDG saline), or by addition of amiloride to the extracellular solution. This current was abolished either by 500 microM SITS (4,4'-diisothiocyanatostilbene-2-2'-disulfonic acid) or 500 microM DPC (diphenylamine-2-carboxylic acid) a cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel blocker, identifying it as a Cl(-) current. RT-PCR identified the presence of ENaC and CFTR transcripts. CFTR blockade reduced cell proliferation, suggesting that this channel plays a physiological role in regulation of S17 cell proliferation.
Authors:
Henrique B Silva; Emiliano Medei; Deivid C Rodrigues; Edson Rondinelli; Norma A S Almeida; Regina C S Goldenberg; Antonio C Campos de Carvalho; Jos? H M Nascimento
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  223     ISSN:  1097-4652     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-03-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  244-51     Citation Subset:  IM    
Copyright Information:
J. Cell. Physiol. 223: 244-251, 2010. (c) 2009 Wiley-Liss, Inc.
Affiliation:
UFRJ, IBCCF, Laborat?rio de Eletrofisiologia Card?aca Antonio Paes de Carvalho, Rio de Janeiro, RJ, Brazil.
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MeSH Terms
Descriptor/Qualifier:
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / pharmacology
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / pharmacology
Amiloride / pharmacology
Animals
Anthranilic Acids / pharmacology
Barium / metabolism
Bone Marrow Cells / drug effects,  metabolism*
Calcium / metabolism*
Calcium Channel Agonists / pharmacology
Calcium Channel Blockers / pharmacology
Calcium Channels, L-Type / drug effects,  genetics,  metabolism*
Cell Line
Cell Proliferation*
Chlorides / metabolism*
Cystic Fibrosis Transmembrane Conductance Regulator / drug effects,  genetics,  metabolism*
Epithelial Sodium Channel / metabolism
Kinetics
Membrane Potentials
Mice
Nifedipine / pharmacology
Patch-Clamp Techniques
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sodium / metabolism
Sodium Channel Blockers / pharmacology
Stromal Cells / drug effects,  metabolism*
Chemical
Reg. No./Substance:
0/Anthranilic Acids; 0/Calcium Channel Agonists; 0/Calcium Channel Blockers; 0/Calcium Channels, L-Type; 0/Chlorides; 0/Epithelial Sodium Channel; 0/RNA, Messenger; 0/Sodium Channel Blockers; 126880-72-6/Cystic Fibrosis Transmembrane Conductance Regulator; 21829-25-4/Nifedipine; 2609-46-3/Amiloride; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 71145-03-4/3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; 7440-23-5/Sodium; 7440-39-3/Barium; 7440-70-2/Calcium; 91-40-7/fenamic acid

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