Document Detail


Voltage controlled nano-injection system for single-cell surgery.
MedLine Citation:
PMID:  22899383     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Manipulation and analysis of single cells is the next frontier in understanding processes that control the function and fate of cells. Herein we describe a single-cell injection platform based on nanopipettes. The system uses scanning microscopy techniques to detect cell surfaces, and voltage pulses to deliver molecules into individual cells. As a proof of concept, we injected adherent mammalian cells with fluorescent dyes.
Authors:
R Adam Seger; Paolo Actis; Catherine Penfold; Michelle Maalouf; Boaz Vilozny; Nader Pourmand
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-08-16
Journal Detail:
Title:  Nanoscale     Volume:  4     ISSN:  2040-3372     ISO Abbreviation:  Nanoscale     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-14     Completed Date:  2013-01-22     Revised Date:  2013-08-01    
Medline Journal Info:
Nlm Unique ID:  101525249     Medline TA:  Nanoscale     Country:  England    
Other Details:
Languages:  eng     Pagination:  5843-6     Citation Subset:  IM    
Affiliation:
Department of Biomolecular Engineering, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA 95064, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Cell Membrane / metabolism
Electricity*
Fibroblasts / metabolism
Fluorescent Dyes / chemistry,  metabolism
Humans
Microscopy, Electron, Scanning
Nanotechnology / instrumentation*
Grant Support
ID/Acronym/Agency:
P01 HG000205/HG/NHGRI NIH HHS; P01-35HG000205/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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