Document Detail


Volatile organic compound specific detection by electrochemical signals using a cell-based sensor.
MedLine Citation:
PMID:  18239432     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A cell-based in vitro exposure system was developed to determine whether oxidative stress plays a role in the cytotoxic effects of volatile organic compounds (VOCs) such as benzene, toluene, xylene, and chlorobenzene, using human epithelial HeLa cells. Thin films based on cysteine-terminated synthetic oligopeptides were fabricated for immobilization of the HeLa cells on a gold (Au) substrate. In addition, an immobilized cell-based sensor was applied to the electrochemical detection of the VOCs. Layer formation and immobilization of the cells were investigated with surface plasmon resonance (SPR), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The adhered living cells were exposed to VOCs; this caused a change in the SPR angle and the VOC-specific electrochemical signal. In addition, VOC toxicity was found to correlate with the degree of nitric oxide (NO) generation and EIS. The primary reason for the marked increase in impedance was the change of aqueous electrolyte composition as a result of cell responses. The p53 and NF-kappaB downregulation were closely related to the magnitude of growth inhibition associated with increasing concentrations of each VOC. Therefore, the proposed cell immobilization method, using a self-assembly technique and VOC-specific electrochemical signals, can be applied to construct a cell microarray for onsite VOC monitoring.
Authors:
Sang Gwi Chung; Jo Chun Kim; Chong-Ho Park; Woong-Shick Ahn; Yong-Wan Kim; Jeong-Woo Choi
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of microbiology and biotechnology     Volume:  18     ISSN:  1017-7825     ISO Abbreviation:  J. Microbiol. Biotechnol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-02-01     Completed Date:  2008-05-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9431852     Medline TA:  J Microbiol Biotechnol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  145-52     Citation Subset:  IM    
Affiliation:
Department of Environmental Engineering, KunKuk University, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Biosensing Techniques / methods*
Cells, Immobilized
Electric Impedance
Electrochemistry / methods*
Hela Cells
Humans
Hydrocarbons, Aromatic* / analysis,  pharmacology
Oxidative Stress / drug effects*
Spectrum Analysis
Surface Plasmon Resonance
Toxicity Tests / methods*
Volatilization
Chemical
Reg. No./Substance:
0/Hydrocarbons, Aromatic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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