| Volatile anesthetics increase intracellular calcium in cerebrocortical and hippocampal neurons. | |
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MedLine Citation:
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PMID: 10201687 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: An increase in intracellular calcium concentration ([Ca2+]i) in neurons has been proposed as an important effect of volatile anesthetics, because they alter signaling pathways that influence neurotransmission. However, the existing data for anesthetic-induced increases in [Ca2+]i conflict. METHODS: Changes in [Ca2+]i were measured using fura-2 fluorescence spectroscopy in rat cortical brain slices at 90, 185, 370, and 705 microM isoflurane. To define the causes of an increase in [Ca2+]i, slices were studied in Ca2+-free medium, in the presence of Ca2+-channel blockers, and in the presence of the Ca2+-release inhibitor azumolene. The authors compared the effect of the volatile anesthetic with that of the nonanesthetic compound 1,2-dichlorohexafluorocyclobutane. Single-dose experiments in CA1 neurons in hippocampal slices with halothane (360 microM) and in acutely dissociated CA1 neurons with halothane (360 microM) and isoflurane (445 microM) also were performed. RESULTS: Isoflurane at 0.5, 1, and 2 minimum alveolar concentrations increased basal [Ca2+]i in cortical slices in a dose-dependent manner (P < 0.05). This increase was not altered by Ca2+-channel blockers or Ca2+-free medium but was reduced 85% by azumolene. The nonanesthetic 1,2-dichlorohexafluorocyclobutane did not increase [Ca2+]i. In dissociated CA1 neurons, isoflurane reversibly increased basal [Ca2+]i by 15 nM (P < 0.05). Halothane increased [Ca2+]i in dissociated CA1 neurons and CA1 neurons in hippocampal slices by approximately 30 nM (P < 0.05). CONCLUSIONS: (1) Isoflurane and halothane reversibly increase [Ca2+]i in isolated neurons and in neurons within brain slices. (2) The increase in [Ca2+]i is caused primarily by release from intracellular stores. (3) Increases in [Ca2+]i occur with anesthetics but not with the nonanesthetic 1,2-dichlorohexafluorocyclobutane. |
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Authors:
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C H Kindler; H Eilers; P Donohoe; S Ozer; P E Bickler |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Anesthesiology Volume: 90 ISSN: 0003-3022 ISO Abbreviation: Anesthesiology Publication Date: 1999 Apr |
Date Detail:
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Created Date: 1999-04-21 Completed Date: 1999-04-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 1300217 Medline TA: Anesthesiology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1137-45 Citation Subset: AIM; IM |
Affiliation:
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Department of Anesthesia, University of California, San Francisco 94143-0542, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anesthetics, Inhalation
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pharmacology* Animals Calcium / metabolism* Cerebral Cortex / drug effects*, metabolism Halothane / pharmacology* Hippocampus / drug effects*, metabolism Isoflurane / pharmacology* Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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R29 GM 55212/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anesthetics, Inhalation; 151-67-7/Halothane; 26675-46-7/Isoflurane; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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