Document Detail

Vitrification vs. slow cooling protocol using embryos cryopreserved in the 5th or 6th day after oocyte retrieval and IVF outcomes.
MedLine Citation:
PMID:  20529821     Owner:  NLM     Status:  MEDLINE    
Modifying cryopreservation protocols may be seen as a way to simplify cryobanking procedure and increase satisfying outcomes. The aim of the study was to evaluate the influence of slow cooling protocol and vitrification on IVF outcomes using embryos preserved in the 5th or 6th day after oocyte retrieval. The study compared 2 groups of human embryos underwent slow cooling protocol (n=189) and vitrification (n=58). All embryos were cryopreserved in the 5th or 6th day after oocyte retrieval. Pre- and postfreezing embryo evaluation was performed in 2 or 3 steps scale, respectively. The study evaluates the effectiveness of two freezing methods and influence of the freezing day, pre- and postfreezing embryo grading on clinical pregnancy rate. Study showed higher pregnancy rate after vitrification (50.4%) than slow cooling protocol (25.9%). Significantly higher pregnancy rate was observed, when embryo preserved in the 5th day after oocyte retrieval (50.3%) than in the 6th day (22.7%). Postfreezing embryos evaluation showed that high quality blastocysts gave nearly four times better pregnancy outcomes than the ones evaluated as poor quality, and three times better than the ones evaluated as moderate. Prospective trials are needed to evaluate pregnancy and neonatal outcomes after vitrification. The number of controlled studies concerning vitrification has not been large enough, yet.
Paweł Kuć; Agnieszka Kuczyńska; Bozena Stankiewicz; Piotr Sieczyński; Joanna Matysiak; Waldemar Kuczyński
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society     Volume:  48     ISSN:  1897-5631     ISO Abbreviation:  Folia Histochem. Cytobiol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-06-10     Completed Date:  2010-09-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8502651     Medline TA:  Folia Histochem Cytobiol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  84-8     Citation Subset:  IM    
Department of Perinatology, Medical University of Białystok, Poland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blastocyst / cytology
Cold Temperature*
Cryopreservation / methods*
Embryo Culture Techniques / methods*
Embryo, Mammalian / physiology*
Fertilization in Vitro / methods*
Logistic Models
Oocyte Retrieval / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Correlation between hepatocyte growth factor receptor and vascular endothelial growth factor-A in br...
Next Document:  Expression of basal cell marker revealed by RAM11 antibody during epithelial regeneration in rabbits...