Document Detail


Vitrification of in vitro matured oocytes of Mangalica and Large White pigs.
MedLine Citation:
PMID:  18828491     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The breeding of Mangalica, a native pig breed in Hungary, had been started in 1833, but this pig breed almost became extinct in Hungary in the past decades. In 1991, the number of sows was only 200. Although in these days the existing Mangalica population consists of more than 6000 animals representing different colour variations, the preservation of this traditional pig breed is still very important. Vitrification is a potential tool for the preservation of gametes and embryos of these animals. The aim of this study was to investigate the effects of vitrification on the developmental competence of Mangalica (M) and Large White (LW) oocytes following fertilisation. The oocytes were vitrified by the Open Pulled Straw (OPS) method using different concentrations of ethylene glycol and dimethyl sulphoxide as cryoprotectants. After rehydration the oocytes underwent in vitro fertilisation; the resultant zygotes were then cultured in vitro for four days to assess embryonic development. In the first experiment, in vitro maturation of M and LW oocytes was compared. No significant difference was observed in the nuclear maturation rate of LW and M oocytes. In the second experiment, the sensitivity of oocytes to vitrification was examined by evaluating oocyte morphology after thawing. A higher percentage of LW oocytes showed normal morphology compared to M oocytes, indicating that Mangalica oocytes are more sensitive to cryoprotectants than Large White oocytes. After warming and in vitro fertilisation, more than 50% of the oocytes started embryonic development and by the end of the incubation period morula stage embryos had developed in both groups. The results show that the OPS vitrification technique is well suited to preserve Mangalica oocytes and from these oocytes morula embryos can be produced.
Authors:
Erika Varga; Erzsébet Gajdócsi; Brigitta Petz Makkosné; Ildikó Salamon; Agnes Bali Papp
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta veterinaria Hungarica     Volume:  56     ISSN:  0236-6290     ISO Abbreviation:  Acta Vet. Hung.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-10-02     Completed Date:  2008-11-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406376     Medline TA:  Acta Vet Hung     Country:  Hungary    
Other Details:
Languages:  eng     Pagination:  399-410     Citation Subset:  IM    
Affiliation:
Institute of Animal Sciences, University of West Hungary, H-9200 Mosonmagyaróvár, Vár 4, Hungary. vargaera81@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Breeding / methods*
Conservation of Natural Resources
Cryopreservation / instrumentation,  methods,  veterinary*
Cryoprotective Agents / pharmacology*
Dose-Response Relationship, Drug
Female
Fertilization in Vitro / instrumentation,  methods,  veterinary*
Oocytes / cytology,  growth & development,  physiology*
Pregnancy
Swine / physiology*
Chemical
Reg. No./Substance:
0/Cryoprotective Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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