Document Detail


Vitrification can modify embryo cleavage stage after warming. Should we change endometrial preparation?
MedLine Citation:
PMID:  23179383     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Studies have shown that embryo metabolism and cell cleavage after warming vitrified embryos is faster than after thawing frozen embryos. We study vitrified embryo transfer (VET) results depending on the developmental stage of warmed embryos and the duration of progesterone treatment before embryo transfer.
METHODS: We designed a prospective study, patients were randomized in two groups, starting progesterone three (D + 3) or four days (D + 4) before embryo transfer. We recruited 88 patients with embryos vitrified on day 3.
RESULTS: We didn't find statitistical differences in pregnancy rate when we transferred embryos in D + 3 vs D + 4 (38.2 % vs 40.5 % p ≥ 0.05). The day after warming, 54.6 % of embryos had developed to morula or early blastocyst, 32.4 % to cleavage stage and 13 % didn't cleave. Transfers were with morula/blastocysts stage embryos (52.1 %; n:37), cleavage stage embryos (18.3 %; n:13) or mixed (29.6 %; n:21). Implantation rate was significantly higher in morula/blastocyst stage than in cleavage stage or mixed transfers (44 %, 22 % and 16.3 %; p = 0.011). Pregnancy and implantation rates were significantly higher in morula/blastocyst transfers on D + 4 than on D + 3 (68.7 % and 64.7 % vs 33.3 %, and 33.3 %, p = 0.033 and p = 0.034).
CONCLUSIONS: Our findings suggest that a majority of embryos will develop to morula/blastocyst stage after warming. VET results with morula/blastocysts, and after four days of progesterone supplementation, are better than with cleavage stage embryos.
Authors:
R Cercas; C Villas; I Pons; C Braña; S Fernandez-Shaw
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Publication Detail:
Type:  Journal Article; Randomized Controlled Trial     Date:  2012-11-22
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  29     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-24     Completed Date:  2013-07-12     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1363-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Blastocyst / cytology,  physiology
Cleavage Stage, Ovum / cytology,  physiology
Cryopreservation / methods*
Embryo Culture Techniques*
Embryo Implantation
Embryo Transfer / methods*
Embryonic Development
Female
Humans
Morula / cytology,  physiology
Pregnancy
Pregnancy Rate
Prospective Studies
Vitrification*
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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