Document Detail

Vitreoscilla hemoglobin promoter is not responsive to nitrosative and oxidative stress in Escherichia coli.
MedLine Citation:
PMID:  12855179     Owner:  NLM     Status:  MEDLINE    
The Vitreoscilla hemoglobin gene (vhb) is expressed under oxygen-limited conditions via an FNR-dependent mechanism. Furthermore, cAMP-CRP has been implicated in its regulation. Recently, VHb protein has been reported to protect a heterologous host from nitrosative stress. In this study we analyzed the regulation of the Vitreoscilla hemoglobin promoter (Pvhb) in Escherichia coli under nitrosative and oxidative stress conditions. Our results show unambiguously that expression of neither VHb nor chloramphenicol acetyltransferase under the control of Pvhb is induced under the experimental conditions used. Thus, a clear discrepancy between in vivo function, i.e. protection against nitrosative stress, and regulation of gene expression is obvious. The regulation of Pvhb reported here is in clear contrast to the expression pattern of flavohemoglobins from various microorganisms, which are generally induced by nitrosative stress. However, the length of Pvhb is only 146 bp and therefore, we cannot rule out that additional regulatory sequences may be located in the upstream region of Pvhb.
Alexander D Frey; Taija Koskenkorva; Pauli T Kallio
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  224     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-07-11     Completed Date:  2003-09-22     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  127-32     Citation Subset:  IM    
Institute of Biotechnology, ETH Zürich, CH-8093 Zürich, Switzerland.
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MeSH Terms
Bacterial Proteins / genetics*
Chloramphenicol O-Acetyltransferase / genetics
Escherichia coli / genetics*,  metabolism*
Gene Expression Regulation, Bacterial
Hemoglobins / genetics*
Nitrogen / metabolism
Oxidative Stress / physiology*
Promoter Regions, Genetic / physiology
Truncated Hemoglobins
Reg. No./Substance:
0/Bacterial Proteins; 0/Hemoglobins; 0/Truncated Hemoglobins; 104781-86-4/hemoglobin protein, Vitreoscilla; 7727-37-9/Nitrogen; EC O-Acetyltransferase

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