Document Detail

Vitreoretinal surgery using bromphenol blue as a vital stain: evaluation of staining characteristics in humans.
MedLine Citation:
PMID:  17314149     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate the staining characteristics of bromphenol blue used during vitreoretinal surgery in humans.
PATIENTS AND METHODS: 13 patients with epiretinal membranes were included. Before and after surgery a complete clinical examination including best corrected visual acuity, funduscopy, fluorescein angiography, OCT (Stratus), Goldmann perimetry and multifocal ERG as well as photography of the macular area was performed. Bromphenol blue was used in concentrations of 0.2% in most patients. Removed epiretinal tissue was evaluated using electron microscopy.
RESULTS: Using dye concentrations of 0.2% a good demarcation of epiretinal membranes was seen in 11/13 patients. Staining of vitreous remnants at the vitreous base was seen in all patients. No dye-related adverse events were seen during follow-up in the functional tests (VA, ERG, perimetry) performed. Histological evaluation of epiretinal membranes showed unremarkable aspects of epiretinal cellular layers and unremarkable retinal surface of the internal limiting membrane (ILM).
CONCLUSION: Bromphenol blue appears to be a very helpful and safe tool in posterior segment surgery. The staining characteristics need to be further evaluated in prospective study settings and larger numbers of patients.
Christos Haritoglou; Ricarda G Schumann; Rupert Strauss; Siegfried G Priglinger; Aljoscha S Neubauer; Anselm Kampik
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-21
Journal Detail:
Title:  The British journal of ophthalmology     Volume:  91     ISSN:  0007-1161     ISO Abbreviation:  Br J Ophthalmol     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-21     Completed Date:  2007-10-04     Revised Date:  2014-07-29    
Medline Journal Info:
Nlm Unique ID:  0421041     Medline TA:  Br J Ophthalmol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1125-8     Citation Subset:  IM    
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MeSH Terms
Bromphenol Blue / diagnostic use*
Coloring Agents / diagnostic use*
Epiretinal Membrane / pathology,  surgery*
Microscopy, Electron
Middle Aged
Retina / surgery*,  ultrastructure
Staining and Labeling / methods
Vitrectomy / methods
Vitreous Body / surgery*,  ultrastructure
Reg. No./Substance:
0/Coloring Agents; 0R2969YC90/Bromphenol Blue
Comment In:
Br J Ophthalmol. 2007 Sep;91(9):1101-2   [PMID:  17709577 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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