Document Detail

Vitellogenin induction in the endangered goodeid fish Girardinichthys viviparus: vitellogenin characterization and estrogenic effects of polychlorinated biphenyls.
MedLine Citation:
PMID:  16380299     Owner:  NLM     Status:  MEDLINE    
Vitellogenin (VTG) is a widely used biomarker in studies of endocrine disruption induced by xenobiotics such as polychlorinated biphenyls (PCBs). This study evaluates the estrogenic effects of these compounds on the black-fin goodeid Girardinichthys viviparus, an endangered fish species in Mexico with a reduced range of distribution due to pollution of its natural environment. Adult fish born in the laboratory were exposed to half the LC(0) of Inerteen commercial PCB mixture. VTG was determined through an inhibition enzyme-linked immunosorbent assay (ELISA) using a homologous-heterologous system. Male and female fish were killed after 1, 2, 4, 8 and 16 days of exposure. The distal third of each specimen was used for analysis. VTG was obtained from cultured hepatocytes and blood serum of males previously exposed to 17beta-estradiol. VTG molecular mass was 348 kDa. PCBs were found to elicit greater estrogenic effects on VTG induction in males than in females (p<0.05) and sex differences were noted. Time-dependent VTG induction kinetics in males and a stationary phase in females were also observed.
Armando Vega-López; Laura Martínez-Tabche; Maria Lilia Domínguez-López; Ethel García-Latorre; Eva Ramón-Gallegos; Alejandra García-Gasca
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Publication Detail:
Type:  Journal Article     Date:  2005-12-27
Journal Detail:
Title:  Comparative biochemistry and physiology. Toxicology & pharmacology : CBP     Volume:  142     ISSN:  1532-0456     ISO Abbreviation:  Comp. Biochem. Physiol. C Toxicol. Pharmacol.     Publication Date:    2006 Mar-Apr
Date Detail:
Created Date:  2006-03-20     Completed Date:  2006-07-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100959500     Medline TA:  Comp Biochem Physiol C Toxicol Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  356-64     Citation Subset:  IM    
Laboratorio de Toxicología Acuática, Escuela Nacional de Ciencias Biológicas, IPN. Prol. Carpio y Plan de Ayala s/n, Col. Plutarco Elías Calles Casco de Santo Tomás, D.F. CP 11340, México.
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MeSH Terms
Cells, Cultured
Cyprinodontiformes / metabolism*
Endocrine Disruptors / toxicity*
Enzyme-Linked Immunosorbent Assay / methods
Estradiol / administration & dosage,  pharmacology
Hepatocytes / metabolism
Immune Sera / biosynthesis,  immunology
Oncorhynchus mykiss
Polychlorinated Biphenyls / toxicity*
Sex Factors
Time Factors
Vitellogenins / biosynthesis*,  blood,  immunology
Water Pollutants, Chemical / toxicity*
Reg. No./Substance:
0/Endocrine Disruptors; 0/Immune Sera; 0/Polychlorinated Biphenyls; 0/Vitellogenins; 0/Water Pollutants, Chemical; 50-28-2/Estradiol

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