| Vitellogenin assay by enzyme-linked immunosorbant assay as a biomarker of endocrine disruptor chemicals pollution. | |
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MedLine Citation:
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PMID: 19090107 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Research is ongoing to develop screening and testing programmes for endocrine disrupting effects of new chemicals and in the focus of this development are the fish test species common carp (Cyprinus carpio). In this study we have developed quantitative enzyme linked immunosorbent assays (ELISA) for VTS in common carp. The working range of the ELISA was 11.25 to 2000 ng mL(-1) (75-25% specific binding/maximum antibody binding [B/B0]) with a 50% B/B0 intra- and interassay variation of 3.9% (n=10) and 12.5% (n=30), respectively. This ELISA is capable of detecting VTG as low as 6 ng mL(-1) and can accurately detect VTG in even 10 microL of plasma. The ELISA was applied to measurement of VTG production by male carp (Cyprinous carpio, Cyprinidae) fish exposure to ethynylestradiol. The results showed that the amount of VTG produced in plasma of exposed fish increased in logaritmic order comparing to the control group and the ELISA described here could be used as an indicator of water pollution to estrogenic pollutants. |
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Authors:
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Mansour Ebrahimi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Pakistan journal of biological sciences: PJBS Volume: 10 ISSN: 1028-8880 ISO Abbreviation: Pak. J. Biol. Sci. Publication Date: 2007 Sep |
Date Detail:
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Created Date: 2008-12-18 Completed Date: 2009-01-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101247723 Medline TA: Pak J Biol Sci Country: Pakistan |
Other Details:
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Languages: eng Pagination: 3109-14 Citation Subset: IM |
Affiliation:
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Green Research Center, University of QOM, QOM, Iran. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers / chemistry* Carps Dose-Response Relationship, Drug Endocrine System / drug effects*, metabolism* Environmental Monitoring Enzyme-Linked Immunosorbent Assay / methods* Estrogens / metabolism Ethinyl Estradiol / pharmacology Male Models, Biological Reproducibility of Results Vitellogenins / analysis* Water Pollutants, Chemical / toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Estrogens; 0/Vitellogenins; 0/Water Pollutants, Chemical; 57-63-6/Ethinyl Estradiol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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