| Vitamin A deficiency alters rat lung alveolar basement membrane: reversibility by retinoic acid. | |
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MedLine Citation:
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PMID: 19269151 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vitamin A is essential for lung development and pulmonary cell differentiation and its deficiency results in alterations of lung structure and function. Basement membranes (BMs) are also involved in those processes, and retinoic acid, the main biologically active form of vitamin A, influences the expression of extracellular matrix macromolecules. Therefore, we have analyzed the ultrastructure and collagen content of lung alveolar BM in growing rats deficient in vitamin A and the recovering effect of all-trans retinoic acid. Male weanling pups were fed a retinol-adequate or -deficient diet until they were 60 days old. A group of vitamin A-deficient pups were recovered by daily intraperitoneal injections of all-trans retinoic acid for 10 days. Alveolar BM in vitamin A-deficient rats doubled its thickness and contained irregularly scattered collagen fibrils. Immunocytochemistry revealed that these fibrils were composed of collagen I. Total content of both collagen I protein and its mRNA was greater in vitamin-deficient lungs. In agreement with the greater size of the BM the amount of collagen IV was also increased. Proinflammatory cytokines, IL-1alpha, IL-1beta and TNF-alpha, did not change, but myeloperoxidase and TGF-beta1 were increased. Treatment of vitamin A-deficient rats with retinoic acid reversed all the alterations, but the BM thickness recovered only partially. Retinoic acid recovering activity occurred in the presence of increasing oxidative stress. In conclusion, vitamin A deficiency results in alterations of the structure and composition of the alveolar BM which are probably mediated by TGF-beta1 and reverted by retinoic acid. These alterations could contribute to the impairment of lung function and predispose to pulmonary disease. |
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Authors:
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Guillermo Esteban-Pretel; M Pilar Mar?n; Jaime Renau-Piqueras; Teresa Barber; Joaqu?n Timoneda |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-09 |
Journal Detail:
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Title: The Journal of nutritional biochemistry Volume: 21 ISSN: 1873-4847 ISO Abbreviation: J. Nutr. Biochem. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-22 Completed Date: 2010-05-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9010081 Medline TA: J Nutr Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 227-36 Citation Subset: IM |
Copyright Information:
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2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Departamento de Bioqu?mica y Biolog?a Molecular, Facultad de Farmacia, Universidad de Valencia, 46100-Burjassot, Valencia, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Basement Membrane / drug effects*, ultrastructure Collagen Type I / genetics, metabolism Collagen Type IV / metabolism Gene Expression Regulation / drug effects Immunohistochemistry Interleukins / metabolism Lung / metabolism, pathology Male Malondialdehyde / metabolism Oxidative Stress / drug effects Peroxidase / metabolism Protein Subunits / genetics, metabolism Pulmonary Alveoli / drug effects*, ultrastructure RNA, Messenger / metabolism Rats Rats, Wistar Transforming Growth Factor beta1 / metabolism Tretinoin / adverse effects, blood, metabolism, therapeutic use* Tumor Necrosis Factor-alpha / metabolism Vitamin A Deficiency / drug therapy, pathology*, physiopathology* |
| Chemical | |
Reg. No./Substance:
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0/Collagen Type I; 0/Collagen Type IV; 0/Interleukins; 0/Protein Subunits; 0/RNA, Messenger; 0/Transforming Growth Factor beta1; 0/Tumor Necrosis Factor-alpha; 302-79-4/Tretinoin; 542-78-9/Malondialdehyde; EC 1.11.1.7/Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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