Document Detail

Inhibitory effects of vitamin E on UVB-induced apoptosis of chicken embryonic fibroblasts.
MedLine Citation:
PMID:  21054279     Owner:  NLM     Status:  MEDLINE    
Apoptosis research has been focused on several model species in the past decades, whereas studies concerned with non-mammalian vertebrate, particularly birds, have rarely been involved. In accord with requirements to expand the biodiversity of apoptotic research, a chicken embryonic fibroblasts model involving UVB (ultraviolet B) as the death stimulus was established through primary explantation and serial passage. Myriads of antioxidants can inhibit UVB-induced apoptosis by virtue of scavenging reactive oxygen species. To improve our understanding of the possible anti-apoptotic effects and mechanisms of Vitamin E against UVB-induced apoptosis in chicken embryonic fibroblasts, cells treated with Vitamin E after UVB irradiation were stained with AO/EB and Fluo-3/AM to visualize chromatin distribution and calcium homoeostasis, respectively. They were also analysed by flow cytometry to detect mitochondrial transmembrane potential, and cell cycle progression and apoptotic rates were recorded. RT-PCR was used to analyse the expression of some apoptosis-related genes. Typical apoptotic events, including cell shrinkage, blebbing and nuclear condensation, occurred after radiation. In the presence of Vitamin E following irradiation, apoptotic cells were reduced. Ca2+ release was temporarily prevented, and cell cycle arrest at S/G2 checkpoint had almost completely reverted to normal. fas decreased, while procaspase-3 remained nearly unchanged with and without Vitamin E, and bcl2/bax ratio was up-regulated, indicating possible anti-apoptotic mechanisms through the mitochondrial pathway. This new investigation of an apoptosis model involving chicken embryonic fibroblasts expands the database of knowledge across a wider spectrum of vertebrate species.
Dapeng P Jin; Chunying Li; Yimei Cong; Hongjian Yang; Wenxiu X Zhang; Weijun Guan; Yuehui Ma
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  35     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-18     Completed Date:  2011-07-14     Revised Date:  2012-08-02    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  381-9     Citation Subset:  IM    
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People’s Republic of China.
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MeSH Terms
Antioxidants / metabolism*
Apoptosis / radiation effects*
Calcium / metabolism
Cells, Cultured
Chick Embryo
Fibroblasts / cytology*,  metabolism,  radiation effects
Flow Cytometry
In Situ Nick-End Labeling
Reverse Transcriptase Polymerase Chain Reaction
Ultraviolet Rays / adverse effects*
Vitamin E / metabolism*
Reg. No./Substance:
0/Antioxidants; 1406-18-4/Vitamin E; 7440-70-2/Calcium

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