Document Detail


Vitamin E deficiency decreases long-chain PUFA in zebrafish (Danio rerio).
MedLine Citation:
PMID:  22013196     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
α-Tocopherol is a required, lipid-soluble antioxidant that protects PUFA. We hypothesized that α-tocopherol deficiency in zebrafish compromises PUFA status. Zebrafish were fed for 1 y either an α-tocopherol-sufficient (E+; 500 mg α-tocopherol/kg) or -deficient (E-; 1.1 mg α-tocopherol/kg) diet containing α-linolenic (ALA) and linoleic (LA) acids but without arachidonic acid (ARA), EPA, or DHA. Vitamin E deficiency in zebrafish decreased by ~20% (n-6) (P < 0.05) and (n-3) (P < 0.05) PUFA and increased the (n-6):(n-3) PUFA ratio (P < 0.05). In E- compared to E+ females, long chain-PUFA status was impaired, as assessed by a ~60% lower DHA:ALA ratio (P < 0.05) and a ~50% lower ARA:LA ratio (P < 0.05). fads2 (P < 0.05) and elovl2 (P < 0.05) mRNA expression was doubled in E- compared to E+ fish. Thus, inadequate vitamin E status led to a depletion of PUFA that may be a result of either or both increased lipid peroxidation and an impaired ability to synthesize sufficient PUFA, especially (n-3) PUFA.
Authors:
Katie M Lebold; Donald B Jump; Galen W Miller; Charlotte L Wright; Edwin M Labut; Carrie L Barton; Robert L Tanguay; Maret G Traber
Related Documents :
19101516 - Pallidol, a resveratrol dimer from red wine, is a selective singlet oxygen quencher.
19319346 - Phenol accumulation related to resistance in tomato to infection by root-knot and lesio...
7507456 - Evaluation of the antioxidant actions of ferulic acid and catechins.
25383786 - Bile acid pool dynamics in progressive familial intrahepatic cholestasis with partial e...
8481386 - In vivo conversion of recombinant human proapolipoprotein ai (rh-met-proapo ai) to apol...
14713846 - Calcium oxalate crystal adherence in the rat bladder: restoration of anti-adherence aft...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-10-19
Journal Detail:
Title:  The Journal of nutrition     Volume:  141     ISSN:  1541-6100     ISO Abbreviation:  J. Nutr.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-21     Completed Date:  2012-01-12     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2113-8     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acid / administration & dosage
Diet*
Docosahexaenoic Acids / analysis
Fatty Acids, Unsaturated / analysis*,  metabolism*
Female
Gene Expression Regulation / drug effects
Linoleic Acid / administration & dosage,  analysis
Lipid Peroxidation
Male
RNA / isolation & purification
RNA, Messenger / metabolism
Real-Time Polymerase Chain Reaction
Vitamin E Deficiency / metabolism*
Zebrafish
alpha-Linolenic Acid / administration & dosage,  analysis
alpha-Tocopherol / administration & dosage
Grant Support
ID/Acronym/Agency:
DK43220/DK/NIDDK NIH HHS; HD062109/HD/NICHD NIH HHS; P30 ES000210/ES/NIEHS NIH HHS; P30 ES000210/ES/NIEHS NIH HHS; R01 DK043220/DK/NIDDK NIH HHS; R01 HD062109/HD/NICHD NIH HHS; RC4 ES019764/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids, Unsaturated; 0/RNA, Messenger; 0RBV727H71/alpha-Linolenic Acid; 25167-62-8/Docosahexaenoic Acids; 27YG812J1I/Arachidonic Acid; 63231-63-0/RNA; 9KJL21T0QJ/Linoleic Acid; H4N855PNZ1/alpha-Tocopherol
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Intermittent bolus feeding has a greater stimulatory effect on protein synthesis in skeletal muscle ...
Next Document:  Severe postnatal iron deficiency alters emotional behavior and dopamine levels in the prefrontal cor...