Document Detail


Vitamin A deficiency results in meiotic failure and accumulation of undifferentiated spermatogonia in prepubertal mouse testis.
MedLine Citation:
PMID:  20881313     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vitamin A (retinol) is required for maintenance of adult mammalian spermatogenesis. In adult rodents, vitamin A withdrawal is followed by a loss of differentiated germ cells within the seminiferous epithelium and disrupted spermatogenesis that can be restored by vitamin A replacement. However, whether vitamin A plays a role in the differentiation and meiotic initiation of germ cells during the first round of mouse spermatogenesis is unknown. In the present study, we found that vitamin A depletion markedly decreased testicular expression of the all-trans retinoic acid-responsive gene, Stra8, and caused meiotic failure in prepubertal male mice lacking lecithin:retinol acyltransferase (Lrat), encoding for the major enzyme in liver responsible for the formation of retinyl esters. Rather than undergoing normal differentiation, germ cells accumulated in the testes of Lrat(-/-) mice maintained on a vitamin A-deficient diet. These results, together with our previous observations that germ cells fail to enter meiosis and remain undifferentiated in embryonic vitamin A-deficient ovaries, support the hypothesis that vitamin A regulates the initiation of meiosis I of both oogenesis and spermatogenesis in mammals.
Authors:
Hui Li; Krzysztof Palczewski; Wolfgang Baehr; Margaret Clagett-Dame
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-09-29
Journal Detail:
Title:  Biology of reproduction     Volume:  84     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-21     Completed Date:  2011-04-12     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  336-41     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706-1544, USA.
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MeSH Terms
Descriptor/Qualifier:
Acyltransferases / deficiency
Animals
Cell Differentiation
Down-Regulation
Female
Male
Meiosis*
Mice
Mice, Knockout
Proteins / metabolism
Sexual Maturation*
Spermatogenesis
Spermatogonia / pathology*
Testis / pathology*
Vitamin A / metabolism
Vitamin A Deficiency / pathology*,  physiopathology*
Grant Support
ID/Acronym/Agency:
EY008061/EY/NEI NIH HHS; EY019298/EY/NEI NIH HHS; P30 EY014800-07/EY/NEI NIH HHS; R01 EY008061-26/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Proteins; 0/Stra8 protein, mouse; 11103-57-4/Vitamin A; EC 2.3.-/Acyltransferases; EC 2.3.1.-/lecithin-retinol acyltransferase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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