| Vitamin A deficiency results in meiotic failure and accumulation of undifferentiated spermatogonia in prepubertal mouse testis. | |
MedLine Citation:
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PMID: 20881313 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vitamin A (retinol) is required for maintenance of adult mammalian spermatogenesis. In adult rodents, vitamin A withdrawal is followed by a loss of differentiated germ cells within the seminiferous epithelium and disrupted spermatogenesis that can be restored by vitamin A replacement. However, whether vitamin A plays a role in the differentiation and meiotic initiation of germ cells during the first round of mouse spermatogenesis is unknown. In the present study, we found that vitamin A depletion markedly decreased testicular expression of the all-trans retinoic acid-responsive gene, Stra8, and caused meiotic failure in prepubertal male mice lacking lecithin:retinol acyltransferase (Lrat), encoding for the major enzyme in liver responsible for the formation of retinyl esters. Rather than undergoing normal differentiation, germ cells accumulated in the testes of Lrat(-/-) mice maintained on a vitamin A-deficient diet. These results, together with our previous observations that germ cells fail to enter meiosis and remain undifferentiated in embryonic vitamin A-deficient ovaries, support the hypothesis that vitamin A regulates the initiation of meiosis I of both oogenesis and spermatogenesis in mammals. |
Authors:
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Hui Li; Krzysztof Palczewski; Wolfgang Baehr; Margaret Clagett-Dame |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-09-29 |
Journal Detail:
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Title: Biology of reproduction Volume: 84 ISSN: 1529-7268 ISO Abbreviation: Biol. Reprod. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-21 Completed Date: 2011-04-12 Revised Date: 2013-07-03 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: United States |
Other Details:
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Languages: eng Pagination: 336-41 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706-1544, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acyltransferases
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deficiency Animals Cell Differentiation Down-Regulation Female Male Meiosis* Mice Mice, Knockout Proteins / metabolism Sexual Maturation* Spermatogenesis Spermatogonia / pathology* Testis / pathology* Vitamin A / metabolism Vitamin A Deficiency / pathology*, physiopathology* |
| Grant Support | |
ID/Acronym/Agency:
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EY008061/EY/NEI NIH HHS; EY019298/EY/NEI NIH HHS; P30 EY014800-07/EY/NEI NIH HHS; R01 EY008061-26/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Proteins; 0/Stra8 protein, mouse; 11103-57-4/Vitamin A; EC 2.3.-/Acyltransferases; EC 2.3.1.-/lecithin-retinol acyltransferase |
| Comments/Corrections | |
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