Document Detail

Vitamin D receptors in breast cancer cells.
MedLine Citation:
PMID:  7881099     Owner:  NLM     Status:  MEDLINE    
1,25-(OH)2-Vitamin D3, the active metabolite of vitamin D, is a secosteroid hormone with known differentiating activity in leukemic cells. Studies have demonstrated the presence of vitamin D receptors (VDR) in a wide range of tissues and cell types. Antiproliferative activity of 1,25-(OH)2-vitamin D3 has been documented in osteosarcoma, melanoma, colon carcinoma, and breast carcinoma cells. This study was designed to analyze vitamin D receptor level in breast cancer cells as a marker of differentiation and as a predictor of growth inhibition by 1,25-(OH)2-vitamin D3. VDR messenger RNA was found to be present in relatively high levels in well-differentiated cells and in low levels in poorly differentiated cells. All cell lines had detectable VDR mRNA. Radiolabeled ligand binding assay showed a similar pattern. MCF-7 and T47D cells, which express VDR at moderate levels, showed significant growth inhibition by 10(-9) M1,25-(OH)2-vitamin D3 (p < 0.05). MDA-MB-231 cells, which have very low levels of VDR, demonstrated no growth inhibition by 1,25-(OH)2-vitamin D3 at concentrations up to 10(-6) M. Based on these results it can be stated that VDR expression is lost with de-differentiation and that receptor is essential for the antiproliferative response to 1,25-(OH)2-vitamin D3.
R R Buras; L M Schumaker; F Davoodi; R V Brenner; M Shabahang; R J Nauta; S R Evans
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Breast cancer research and treatment     Volume:  31     ISSN:  0167-6806     ISO Abbreviation:  Breast Cancer Res. Treat.     Publication Date:  1994  
Date Detail:
Created Date:  1995-04-12     Completed Date:  1995-04-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8111104     Medline TA:  Breast Cancer Res Treat     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  191-202     Citation Subset:  IM    
Department of Surgery, Georgetown University, Washington DC.
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MeSH Terms
Adenocarcinoma / chemistry,  pathology
Anticarcinogenic Agents / pharmacology
Breast Neoplasms / chemistry,  pathology*
Calcitriol / pharmacology*
Carcinoma, Ductal, Breast / chemistry,  pathology
Cell Differentiation
Cell Division / drug effects
DNA, Complementary / genetics
Growth Inhibitors / pharmacology*
Neoplasm Proteins / analysis,  physiology*
Pleural Effusion / pathology
Receptors, Calcitriol / analysis,  drug effects*,  physiology
Tumor Cells, Cultured
Tumor Markers, Biological
Reg. No./Substance:
0/Anticarcinogenic Agents; 0/DNA, Complementary; 0/Growth Inhibitors; 0/Neoplasm Proteins; 0/Receptors, Calcitriol; 0/Tumor Markers, Biological; 32222-06-3/Calcitriol

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