Document Detail

Vitamin C reversed malfunction of peripheral blood-derived mononuclear cells in smokers through antioxidant properties.
MedLine Citation:
PMID:  18362440     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Smoking impairs neovascularization, possibly, through the impaired function of peripheral blood-derived mononuclear cells (PB-MNCs). Thus, the mechanism of impaired function of PB-MNCs caused by chronic smoking was examined, and whether vitamin C reversed the malfunction of PB-MNCs in smokers was investigated. METHODS AND RESULTS: The cohort comprised 27 healthy male volunteers (16 smokers and 11 age-matched non-smokers). For evaluation of the colony-forming activity of PB-MNCs, the number of endothelial colony-forming units (e-CFUs) was counted in a culture assay. Migration activity of PB-MNCs was evaluated by the modified Boyden chamber method. In smokers, the number of e-CFUs was reduced to 56% and migratory activity of PB-MNCs to 40% compared with non-smokers (p<0.01). The urinary level of 8-isoprostane, an oxidative stress marker, was greater in smokers than in non-smokers (p<0.05). There was an inverse correlation between migratory activity of PB-MNCs but not between the number of e-CFUs and urinary level of 8-isoprostane. Furthermore, oral administration of vitamin C for 2 weeks ameliorated the impaired migratory activity of PB-MNCs in smokers. CONCLUSION: Chronic smoking impairs the function of PB-MNCs. Smoking-induced oxidative stress may be involved in impaired migratory activity of PB-MNCs.
Yoshiaki Takeshita; Yoshio Katsuki; Yousuke Katsuda; Hisashi Kai; Yutaka Saito; Ken Arima; Ken-Ichiro Sasaki; Tsutomu Imaizumi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Circulation journal : official journal of the Japanese Circulation Society     Volume:  72     ISSN:  1346-9843     ISO Abbreviation:  Circ. J.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-25     Completed Date:  2008-08-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101137683     Medline TA:  Circ J     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  654-9     Citation Subset:  IM    
Division of Cardiovascular Medicine, Department of Medicine, Kurume University School of Medicine, Kurume, Japan.
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MeSH Terms
Antioxidants / pharmacology*
Ascorbic Acid / pharmacology*
Case-Control Studies
Cell Movement / drug effects
Cohort Studies
Colony-Forming Units Assay
Cytokines / blood
Dinoprost / analogs & derivatives,  urine
Endothelial Cells / drug effects,  pathology,  physiology
Leukocytes, Mononuclear / drug effects*,  metabolism*,  pathology
Oxidative Stress
Smoking / blood*,  drug therapy*,  pathology,  urine
Vascular Endothelial Growth Factor A / blood,  pharmacology
Reg. No./Substance:
0/Antioxidants; 0/Cytokines; 0/VEGFA protein, human; 0/Vascular Endothelial Growth Factor A; 27415-26-5/8-epi-prostaglandin F2alpha; 50-81-7/Ascorbic Acid; 551-11-1/Dinoprost

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