| Vitamin C protects human vascular smooth muscle cells against apoptosis induced by moderately oxidized LDL containing high levels of lipid hydroperoxides. | |
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MedLine Citation:
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PMID: 10521368 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vascular cell death is a key feature of atherosclerotic lesions and may contribute to the plaque "necrotic" core, cap rupture, and thrombosis. Oxidatively modified low-density lipoproteins (LDLs) are implicated in the pathogenesis of atherosclerosis, and dietary antioxidants are thought to protect the vasculature against LDL-induced cytotoxicity. Because LDL oxidative modification may vary within atherosclerotic lesions, we examined the effects of defined, oxidatively modified LDL species on human arterial smooth muscle cell apoptosis and the cytoprotective effects of vitamin C. Moderately oxidized LDL (0 to 300 microg protein/mL), which has the highest content of lipid hydroperoxides, induced smooth muscle cell apoptosis within 6 hours, whereas native LDL and mildly and highly oxidized LDL had no effect. Moderately oxidized LDL increased cellular DNA fragmentation, release of fragmented DNA into the culture medium, and annexin V binding and decreased mitochondrial dehydrogenase activity and expression of the antiapoptotic mediator Bcl-x(L). Treatment of cells with native LDL together with the lipid hydroperoxide 13(S)-hydroperoxyoctadeca-9Z,11E-dienoic acid (HPODE, 200 micromol/L, 6 to 24 hours) also induced apoptotic cell death. Pretreatment of smooth muscle cells with vitamin C (0 to 100 micromol/L, 24 hours) attenuated the cytotoxicity and apoptosis induced by both moderately oxidized LDL and HPODE. Our findings suggest that moderately oxidized LDL, with its high lipid hydroperoxide content, rather than mildly or highly oxidized LDL, causes apoptosis of human smooth muscle cells and that vitamin C supplementation may provide protection against plaque instability in advanced atherosclerosis. |
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Authors:
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R C Siow; J P Richards; K C Pedley; D S Leake; G E Mann |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Arteriosclerosis, thrombosis, and vascular biology Volume: 19 ISSN: 1079-5642 ISO Abbreviation: Arterioscler. Thromb. Vasc. Biol. Publication Date: 1999 Oct |
Date Detail:
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Created Date: 1999-10-29 Completed Date: 1999-10-29 Revised Date: 2009-09-29 |
Medline Journal Info:
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Nlm Unique ID: 9505803 Medline TA: Arterioscler Thromb Vasc Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2387-94 Citation Subset: IM |
Affiliation:
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Centre for Cardiovascular Biology and Medicine, GKT School of Biomedical Sciences, King's College, London, UK. rcms2@cam.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Annexin A5
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metabolism Antioxidants / pharmacology* Apoptosis / drug effects* Ascorbic Acid / pharmacology* Cells, Cultured Coloring Agents Cross Reactions Cytotoxins / metabolism DNA Fragmentation Humans Linoleic Acids / metabolism* Lipid Peroxides / metabolism* Lipoproteins, LDL / metabolism* Muscle, Smooth, Vascular / cytology*, metabolism Propidium Proto-Oncogene Proteins c-bcl-2 / analysis, biosynthesis, immunology |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Annexin A5; 0/Antioxidants; 0/Coloring Agents; 0/Cytotoxins; 0/Linoleic Acids; 0/Lipid Peroxides; 0/Lipoproteins, LDL; 0/Proto-Oncogene Proteins c-bcl-2; 0/oxidized low density lipoprotein; 23017-93-8/13-hydroperoxy-9,11-octadecadienoic acid; 36015-30-2/Propidium; 50-81-7/Ascorbic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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