Document Detail


Vitamin C protects human vascular smooth muscle cells against apoptosis induced by moderately oxidized LDL containing high levels of lipid hydroperoxides.
MedLine Citation:
PMID:  10521368     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vascular cell death is a key feature of atherosclerotic lesions and may contribute to the plaque "necrotic" core, cap rupture, and thrombosis. Oxidatively modified low-density lipoproteins (LDLs) are implicated in the pathogenesis of atherosclerosis, and dietary antioxidants are thought to protect the vasculature against LDL-induced cytotoxicity. Because LDL oxidative modification may vary within atherosclerotic lesions, we examined the effects of defined, oxidatively modified LDL species on human arterial smooth muscle cell apoptosis and the cytoprotective effects of vitamin C. Moderately oxidized LDL (0 to 300 microg protein/mL), which has the highest content of lipid hydroperoxides, induced smooth muscle cell apoptosis within 6 hours, whereas native LDL and mildly and highly oxidized LDL had no effect. Moderately oxidized LDL increased cellular DNA fragmentation, release of fragmented DNA into the culture medium, and annexin V binding and decreased mitochondrial dehydrogenase activity and expression of the antiapoptotic mediator Bcl-x(L). Treatment of cells with native LDL together with the lipid hydroperoxide 13(S)-hydroperoxyoctadeca-9Z,11E-dienoic acid (HPODE, 200 micromol/L, 6 to 24 hours) also induced apoptotic cell death. Pretreatment of smooth muscle cells with vitamin C (0 to 100 micromol/L, 24 hours) attenuated the cytotoxicity and apoptosis induced by both moderately oxidized LDL and HPODE. Our findings suggest that moderately oxidized LDL, with its high lipid hydroperoxide content, rather than mildly or highly oxidized LDL, causes apoptosis of human smooth muscle cells and that vitamin C supplementation may provide protection against plaque instability in advanced atherosclerosis.
Authors:
R C Siow; J P Richards; K C Pedley; D S Leake; G E Mann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Arteriosclerosis, thrombosis, and vascular biology     Volume:  19     ISSN:  1079-5642     ISO Abbreviation:  Arterioscler. Thromb. Vasc. Biol.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-10-29     Completed Date:  1999-10-29     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  9505803     Medline TA:  Arterioscler Thromb Vasc Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2387-94     Citation Subset:  IM    
Affiliation:
Centre for Cardiovascular Biology and Medicine, GKT School of Biomedical Sciences, King's College, London, UK. rcms2@cam.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Annexin A5 / metabolism
Antioxidants / pharmacology*
Apoptosis / drug effects*
Ascorbic Acid / pharmacology*
Cells, Cultured
Coloring Agents
Cross Reactions
Cytotoxins / metabolism
DNA Fragmentation
Humans
Linoleic Acids / metabolism*
Lipid Peroxides / metabolism*
Lipoproteins, LDL / metabolism*
Muscle, Smooth, Vascular / cytology*,  metabolism
Propidium
Proto-Oncogene Proteins c-bcl-2 / analysis,  biosynthesis,  immunology
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Annexin A5; 0/Antioxidants; 0/Coloring Agents; 0/Cytotoxins; 0/Linoleic Acids; 0/Lipid Peroxides; 0/Lipoproteins, LDL; 0/Proto-Oncogene Proteins c-bcl-2; 0/oxidized low density lipoprotein; 23017-93-8/13-hydroperoxy-9,11-octadecadienoic acid; 36015-30-2/Propidium; 50-81-7/Ascorbic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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