Document Detail

Vitamin C homeostasis in skeletal muscle cells.
MedLine Citation:
PMID:  15749386     Owner:  NLM     Status:  MEDLINE    
In skeletal muscle, vitamin C not only enhances carnitine biosynthesis but also protects cells against ROS generation induced by physical exercise. The ability to take up both ascorbic and dehydroascorbic acid from the extracellular environment, together with the ability to recycle the intracellular vitamin, maintains high cellular stores of ascorbate. In this study, we examined vitamin C transport and recycling, by using the mouse C2C12 and rat L6C5 muscle cell lines, which exhibit different sensitivity to oxidative stress and GSH metabolism. We found that: (1) both cell lines express SVCT2, whereas SVCT1 is expressed at very low levels only in proliferating L6C5 cells; furthermore L6C5 myoblasts are more efficient in ascorbic acid transport than C2C12 myoblasts; (2) C2C12 cells are more efficient in dehydroascorbic acid transport and ascorbyl free radical/dehydroascorbic acid reduction; (3) differentiation is paralleled by decreased ascorbic acid and dehydroascorbic acid transport and reduction and increased ascorbyl free radical reduction; (4) differentiated cells are more responsive to oxidative stress induced by glutathione depletion; indeed, myotubes showed increased SVCT2 expression and thioredoxin reductase-mediated dehydroascorbic acid reduction. From our data, SVCT2 and NADPH-thioredoxin-dependent DHA reduction appears to belong to an inducible system activated in response to oxidative stress.
Isabella Savini; Maria Valeria Catani; Guglielmo Duranti; Roberta Ceci; Stefania Sabatini; Luciana Avigliano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Free radical biology & medicine     Volume:  38     ISSN:  0891-5849     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-07     Completed Date:  2005-09-26     Revised Date:  2011-01-21    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  898-907     Citation Subset:  IM    
Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy.
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MeSH Terms
Ascorbic Acid / metabolism*
Biological Transport
Cell Differentiation
Cell Line
Dehydroascorbic Acid / metabolism
Glutathione / metabolism
Muscle Fibers, Skeletal / metabolism*
NADP / metabolism
Organic Anion Transporters, Sodium-Dependent / metabolism*
Oxidative Stress / physiology*
Symporters / metabolism*
Thioredoxins / metabolism
Reg. No./Substance:
0/Organic Anion Transporters, Sodium-Dependent; 0/Symporters; 0/sodium-dependent vitamin C transporter; 490-83-5/Dehydroascorbic Acid; 50-81-7/Ascorbic Acid; 52500-60-4/Thioredoxins; 53-59-8/NADP; 70-18-8/Glutathione

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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