Document Detail


Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals.
MedLine Citation:
PMID:  12059148     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To express the antioxidant capacity of plant foods in a more familiar and easily understood manner (equivalent to vitamin C mg/100 g), two stable radical species, ABTS(*)(-) and DPPH(*), commonly used for antioxidant activity measurements, were employed independently to evaluate their efficacies using apple polyphenolic extracts and seven polyphenolic standards including synthetic Trolox. Their antioxidant activities were expressed as vitamin C equivalent antioxidant capacity (VCEAC) in mg/100 g apple or mg/100 mL of the reference chemical compounds in 10 and 30 min using the ABTS(*)(-) and DPPH(*) scavenging assays, respectively. The antioxidant capacity of Gala apples and seven phenolic standards, determined by both ABTS(*)(-) and DPPH(*) scavenging assays, showed a dose-response of the first-order. Fresh Gala apples had a VCEAC of 205.4 +/- 5.6 mg/100 g using the ABTS assay, and the relative VCEACs of phenolic standards were as follows: gallic acid > quercetin > epicatechin > catechin > vitamin C > rutin > chlorogenic acid > Trolox. With the DPPH radical assay, the VCEAC of fresh Gala apples was 136.0 +/- 6.6 mg/100 g, and the relative VCEACs of seven phenolic standards were, in decreasing order, as follows: gallic acid > quercetin > epicatechin > catechin > or = vitamin C > Trolox > rutin > chlorogenic acid. Because the ABTS assay can be used in both organic and aqueous solvent systems, employs a specific absorbance at a wavelength remote from the visible region, and requires a short reaction time, it is a more desirable method than the DPPH assay. Therefore, it is recommended that antioxidant capacity be expressed as vitamin C mg/100 g equivalent (VCEAC) using the ABTS assay.
Authors:
Dae-Ok Kim; Ki Won Lee; Hyong Joo Lee; Chang Yong Lee
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  50     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-06-12     Completed Date:  2002-07-24     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3713-7     Citation Subset:  IM    
Affiliation:
Department of Food Science and Technology, Cornell University, Geneva, New York 14456, USA.
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / pharmacology*
Ascorbic Acid / pharmacology*
Benzothiazoles
Biphenyl Compounds
Catechin / pharmacology
Chlorogenic Acid / pharmacology
Chromans / pharmacology
Free Radical Scavengers
Free Radicals
Gallic Acid / pharmacology
Kinetics
Malus / chemistry
Oxidation-Reduction
Phenols / pharmacology*
Picrates
Plant Extracts / chemistry*
Plants, Edible / chemistry*
Quercetin / pharmacology
Rutin / pharmacology
Sulfonic Acids
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Benzothiazoles; 0/Biphenyl Compounds; 0/Chromans; 0/Free Radical Scavengers; 0/Free Radicals; 0/Phenols; 0/Picrates; 0/Plant Extracts; 0/Sulfonic Acids; 117-39-5/Quercetin; 149-91-7/Gallic Acid; 153-18-4/Rutin; 154-23-4/Catechin; 1898-66-4/2,2-diphenyl-1-picrylhydrazyl; 28752-68-3/2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; 327-97-9/Chlorogenic Acid; 50-81-7/Ascorbic Acid; 56305-04-5/6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid

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