| Vitamin C enhances in vitro and in vivo development of porcine somatic cell nuclear transfer embryos. | |
| | |
MedLine Citation:
|
PMID: 21749856 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The reprogramming of differentiated cells into a totipotent embryonic state through somatic cell nuclear transfer (SCNT) is still an inefficient process. Previous studies revealed that the generation of induced pluripotent stem (iPS) cells from mouse and human fibroblasts could be significantly enhanced with vitamin C treatment. Here, we investigated the effects of vitamin C, to our knowledge for the first time, on the in vitro and in vivo development of porcine SCNT embryos. The rate of blastocyst development in SCNT embryos treated with 50μg/mL vitamin C 15h after activation (36.0%) was significantly higher than that of untreated SCNT embryos (11.5%). The enhanced in vitro development rate of vitamin C-treated embryos was associated with an increased acetylation level of histone H4 lysine 5 and higher Oct4, Sox2 and Klf4 expression levels in blastocysts, as determined by real-time PCR. In addition, treatment with vitamin C resulted in an increased pregnancy rate in pigs. These findings suggest that treatment with vitamin C is beneficial for enhancement of the in vitro and in vivo development of porcine SCNT embryos. |
| | |
Authors:
|
Yongye Huang; Xiaochun Tang; Wanhua Xie; Yan Zhou; Dong Li; Yang Zhou; Jianguo Zhu; Ting Yuan; Liangxue Lai; Daxin Pang; Hongsheng Ouyang |
Related Documents
:
|
18059176 - Ciprofloxacin-induced g2 arrest and apoptosis in tk6 lymphoblastoid cells is not depend... 19109096 - Upon oxidative stress, the antiapoptotic hsp60/procaspase-3 complex persists in mucoepi... 7705936 - Transfected mutant p53 gene increases x-ray-induced cell killing and mutation in human ... 21444956 - Cell death induced by the application of alternating magnetic fields to nanoparticle-lo... 15720826 - Glutathione reductase is expressed at high levels in pancreatic islet cells. 20709786 - The saccharomyces cerevisiae anaphase-promoting complex interacts with multiple histone... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-7-2 |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: - ISSN: 1090-2104 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
|
Created Date: 2011-7-13 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier Inc. |
Affiliation:
|
Jilin Province Key Laboratory of Animal Embryo Engineering, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi An DaLu, Changchun 130062, PR China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Structural and mutational analyses of the receptor binding domain of botulinum D/C mosaic neurotoxin...
Next Document: Herceptin, a recombinant humanized anti-ERBB2 monoclonal antibody, induces cardiomyocyte death.