Document Detail

Vitamin C and copper interactions in guinea-pigs and a study of collagen cross-links.
MedLine Citation:
PMID:  9135375     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was, first to explore metabolic interactions between Cu and ascorbic acid in guinea-pigs, particularly with respect to any possible disadvantages of high ascorbate in the presence of low Cu intakes, and second, to test the hypothesis that variations in ascorbate and/or Cu status might influence collagen cross-linking, either by inducing a change in the crosslinks: hydroxyproline ratio, or by inducing a change in the pyridinoline: deoxypyridinoline crosslinks ratio. Four matched groups, each of eight male weanling Dunkin-Hartley guinea-pigs, were maintained on purified diets containing either no added Cu, or 150 mg Cu/kg diet, and either 0.1 g or 30 g ascorbic acid/kg diet. They were then killed 8 weeks later, and the following indices were measured: body and organ weights; blood haemoglobin; adrenal ascorbate concentrations; Cu concentrations in plasma, liver and femur; superoxide dismutase (EC activity in whole blood and liver; hydroxyproline, pyridinoline and deoxypridinoline in femur and in urine. The principal observations were: Cu intake significantly affected blood and tissue Cu concentrations and superoxide dismutase activity; and ascorbic acid intake significantly affected adrenal ascorbate levels and the deoxypyridinoline: pyridinoline cross-links ratio, especially in bone (femur). There was evidence of a significant interaction between ascorbate and Cu with respect to adrenal and plasma Cu concentrations, blood superoxide dismutase activity and body weights. We conclude that interactions between ascorbate and Cu at the functional level were present but modest, and that a new and potentially powerful functional index of ascorbate status may exist within the deoxypyridinoline:pyridinoline collagen cross-link ratio.
H Tsuchiya; C J Bates
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The British journal of nutrition     Volume:  77     ISSN:  0007-1145     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-05-15     Completed Date:  1997-05-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  315-25     Citation Subset:  IM    
MRC Dunn Nutrition Unit, Cambridge.
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MeSH Terms
Adrenal Glands / chemistry
Amino Acids / analysis,  metabolism*,  urine
Analysis of Variance
Ascorbic Acid / administration & dosage,  analysis,  metabolism*
Bone and Bones / chemistry,  metabolism*
Copper / administration & dosage,  analysis,  metabolism*
Guinea Pigs
Hemoglobins / metabolism
Liver / chemistry,  metabolism
Superoxide Dismutase / analysis
Reg. No./Substance:
0/Amino Acids; 0/Hemoglobins; 50-81-7/Ascorbic Acid; 63800-01-1/pyridinoline; 7440-50-8/Copper; 90032-33-0/deoxypyridinoline; EC Dismutase

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