| Vitamin B6 metabolism in McA-RH7777 cells. | |
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MedLine Citation:
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PMID: 3342390 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The metabolism of vitamin B6 in McA-RH7777 cells has been characterized with respect to pyridoxal 5'-phosphate (PLP) levels, and the activities of pyridoxine (PN) kinase (EC 2.1.7.35) and pyridoxine 5'-phosphate (PNP) oxidase (EC 1.4.3.5). PLP levels (12.4 +/- 4.4 ng/mg protein) were at the lower end of the range found for Morris hepatomas, carcinogen-induced rat hepatomas, and liver from rats fed a PN-deficient diet. PN kinase activity was about one-third of that found in normal rat liver. PNP oxidase appeared to be absent in high-speed supernatants of homogenates prepared from McA-RH7777 cells. The absence of PNP oxidase was supported by enzymatic and immunological data. These findings resemble those found previously for Morris hepatoma 7777. In contrast to rat liver, such preparations caused little or no release of volatile counts upon incubation with either [3H-C4']PN or [3H-C4']PNP. High-speed supernatants of homogenates prepared from both McA-RH7777 cells and Morris hepatoma 7777 were very much less capable than similar preparations from rat liver in converting [G-3H]PN to PLP and pyridoxamine 5'-phosphate. Despite the apparent absence of PNP oxidase, intact confluent or log-phase McA-RH7777 cells were capable of converting [G-3H]PN to PLP and pyridoxamine 5'-phosphate. These findings are discussed in terms of tumor nutrition and vitamin B6 metabolism in a rat hepatoma cell line. |
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Authors:
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N T Meisler; J W Thanassi |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cancer research Volume: 48 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1988 Mar |
Date Detail:
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Created Date: 1988-03-28 Completed Date: 1988-03-28 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1080-5 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Vermont College of Medicine, Burlington 05405. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Liver / metabolism Liver Neoplasms, Experimental / metabolism* Pyridoxal Kinase / analysis Pyridoxal Phosphate / analysis Pyridoxaminephosphate Oxidase / analysis Pyridoxine / metabolism*, pharmacology Rats Tumor Cells, Cultured / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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35878//PHS HHS; 5429-25/16//PHS HHS |
| Chemical | |
Reg. No./Substance:
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54-47-7/Pyridoxal Phosphate; 65-23-6/Pyridoxine; EC 1.4.3.5/Pyridoxaminephosphate Oxidase; EC 2.7.1.35/Pyridoxal Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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