| Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. | |
| | |
MedLine Citation:
|
PMID: 18267078 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The cell-cycle transition from G1 to S phase has been difficult to visualize. We have harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop genetically encoded fluorescent probes for this purpose. These probes effectively label individual G1 phase nuclei red and those in S/G2/M phases green. We were able to generate cultured cells and transgenic mice constitutively expressing the cell-cycle probes, in which every cell nucleus exhibits either red or green fluorescence. We performed time-lapse imaging to explore the spatiotemporal patterns of cell-cycle dynamics during the epithelial-mesenchymal transition of cultured cells, the migration and differentiation of neural progenitors in brain slices, and the development of tumors across blood vessels in live mice. These mice and cell lines will serve as model systems permitting unprecedented spatial and temporal resolution to help us better understand how the cell cycle is coordinated with various biological events. |
| | |
Authors:
|
Asako Sakaue-Sawano; Hiroshi Kurokawa; Toshifumi Morimura; Aki Hanyu; Hiroshi Hama; Hatsuki Osawa; Saori Kashiwagi; Kiyoko Fukami; Takaki Miyata; Hiroyuki Miyoshi; Takeshi Imamura; Masaharu Ogawa; Hisao Masai; Atsushi Miyawaki |
Related Documents
:
|
1391688 - The cell division cycle: a physiologically plausible dynamic model can exhibit chaotic ... 4021508 - Cell cycles and growth laws: the ccc model. 12170768 - Duplication of the genome in normal and cancer cell cycles. 15063858 - Setting the pace: mechanisms tying caulobacter cell-cycle progression to macroscopic ce... 18656478 - Plasmodium yoelii sporozoites modulate cytokine profile and induce apoptosis in murine ... 16943038 - Response of vibrio parahaemolyticus to ethanol shock. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Cell Volume: 132 ISSN: 1097-4172 ISO Abbreviation: Cell Publication Date: 2008 Feb |
Date Detail:
|
Created Date: 2008-02-12 Completed Date: 2008-03-06 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0413066 Medline TA: Cell Country: United States |
Other Details:
|
Languages: eng Pagination: 487-98 Citation Subset: IM |
Affiliation:
|
Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/AB370332; AB370333 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals COS Cells Cell Cycle* Cell Cycle Proteins / genetics, metabolism Cell Line Cells, Cultured Cercopithecus aethiops Cytological Techniques* Fluorescence Hela Cells Humans Luminescent Proteins / genetics, metabolism Mice Mice, Transgenic Microscopy, Confocal Molecular Sequence Data Morphogenesis Neoplasms / pathology Recombinant Fusion Proteins / genetics, metabolism Ubiquitination |
| Chemical | |
Reg. No./Substance:
|
0/CDT1 protein, human; 0/Cell Cycle Proteins; 0/GMNN protein, human; 0/Luminescent Proteins; 0/Recombinant Fusion Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Neuroepithelial stem cell proliferation requires LIS1 for precise spindle orientation and symmetric ...
Next Document: Dialogue between RhoA/ROCK and members of the Par complex in cell polarity.