Document Detail

Visualizing "green oil" in live algal cells.
MedLine Citation:
PMID:  20129108     Owner:  NLM     Status:  MEDLINE    
We report here that BODIPY 505/515, a green lipophilic fluorescent dye, serves as an excellent vital stain for the oil-containing lipid bodies of live algal cells. BODIPY 505/515 vital staining can be used in combination with fluorescent activated cell sorting to detect and isolate algal cells possessing high lipid content.
Mark Scott Cooper; William Robert Hardin; Timothy Wayne Petersen; Rose Ann Cattolico
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-05
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  109     ISSN:  1347-4421     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-04     Completed Date:  2010-04-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  198-201     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2009 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
Department of Biology, University of Washington, Seattle, WA 98195-1800, USA.
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MeSH Terms
Algae / chemistry,  cytology*
Boron Compounds / chemistry*
Flow Cytometry
Fluorescent Dyes / chemistry*
Microscopy, Confocal
Reg. No./Substance:
0/4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; 0/Boron Compounds; 0/Fluorescent Dyes

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