Document Detail


Visualizing fetal mouse great blood vessels by plastic casting.
MedLine Citation:
PMID:  20439420     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this protocol, the great vessels of the mouse are visualized by injecting colored plastic solutions directly into the heart, thereby filling the vessels. The plastic solidifies to create a stable cast that is then revealed by removing the mouse tissues with a caustic. This protocol can be modified for casting the vasculature of other organs by altering the delivery of the liquid plastic. For example, plastic casts of the maternal and fetal vasculature of the placenta can be generated.
Authors:
Chengyu Liu; Jim Martin
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cold Spring Harbor protocols     Volume:  2010     ISSN:  1559-6095     ISO Abbreviation:  Cold Spring Harb Protoc     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-04     Completed Date:  2010-07-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101524530     Medline TA:  Cold Spring Harb Protoc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  pdb.prot5433     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Anatomy / methods*
Animals
Blood Vessels / anatomy & histology*
Corrosion Casting / methods*
Developmental Biology / methods*
Mice
Plastics*
Chemical
Reg. No./Substance:
0/Plastics

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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