Document Detail

Visualizing a circadian clock protein: crystal structure of KaiC and functional insights.
MedLine Citation:
PMID:  15304218     Owner:  NLM     Status:  MEDLINE    
Circadian (daily) biological clocks express characteristics that are difficult to explain by known biochemical mechanisms, and will ultimately require characterizing the structures, functions, and interactions of their molecular components. KaiC is an essential circadian protein in cyanobacteria that forms the core of the KaiABC clock protein complex. We report the crystal structure of the KaiC homohexameric complex at 2.8 A resolution. The structure resembles a double doughnut with a central pore that is partially sealed at one end. The crystal structure reveals ATP binding, inter-subunit organization, a scaffold for Kai-protein complex formation, the location of critical KaiC mutations, and evolutionary relationships to other proteins. A key auto-phosphorylation site on KaiC (T432) is identified from the crystal structure, and mutation of this residue abolishes circadian rhythmicity. The crystal structure of KaiC will be essential for understanding this circadian clockwork and for establishing its links to global gene expression.
Rekha Pattanayek; Jimin Wang; Tetsuya Mori; Yao Xu; Carl Hirschie Johnson; Martin Egli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular cell     Volume:  15     ISSN:  1097-2765     ISO Abbreviation:  Mol. Cell     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-08-12     Completed Date:  2004-09-17     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9802571     Medline TA:  Mol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  375-88     Citation Subset:  IM    
Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA.
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MeSH Terms
Adenosine Triphosphate / physiology
Bacterial Proteins / chemistry*,  genetics,  physiology
Circadian Rhythm / genetics,  physiology*
Circadian Rhythm Signaling Peptides and Proteins
Cyanobacteria / chemistry,  genetics,  physiology
Evolution, Molecular
Protein Structure, Tertiary
Time Factors
Grant Support
Reg. No./Substance:
0/Bacterial Proteins; 0/Circadian Rhythm Signaling Peptides and Proteins; 0/KaiA protein, cyanobacteria; 0/KaiC protein, cyanobacteria; 56-65-5/Adenosine Triphosphate
Erratum In:
Mol Cell. 2004 Sep 10;15(5):841

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