Document Detail

Visualizing acute pain-morphine interaction in descending monoamine nuclei with Fos.
MedLine Citation:
PMID:  19833107     Owner:  NLM     Status:  MEDLINE    
The effect of morphine is often studied in the absence of pain, and it remains poorly understood if and how noxious stimulation may change the activity state of descending pain-modulatory pathways and their response to morphine. Immunohistochemical double-labeling technique with Fos and markers for noradrenergic and serotonergic neurons was used to examine if an intraplantar formalin injection (an acute noxious input) changed the effect of morphine on noradrenergic neurons of the A7 and A5 cell groups, and serotonergic neurons of the nucleus raphe magnus (NRM). Four groups of rats were analyzed: (1) control injected with normal saline subcutaneously, (2) rats treated with FORMALIN into the hind paw 30 min after subcutaneous normal saline injection, (3) rats injected with MORPHINE sulfate subcutaneously, and (4) rats treated with formalin into the hind paw 30 min after subcutaneous morphine injection (morphine/formalin). The average number of total Fos-labeled cells per section was unchanged in all areas of analysis in all treatment groups. However, the percentage of noradrenergic neurons in the A7 and A5 cell groups that contained Fos was significantly increased in the morphine/formalin group compared to all other groups, while no differences were found in serotonin cells in the NRM. In contrast with the view that morphine simply blocks access of nociceptive information to supraspinal brain areas, these data suggest that noxious stimulation has the capacity to modify the actions of morphine on brainstem noradrenergic nuclei, which may participate in descending pain modulation as well as other behavioral responses to pain.
Dusica Bajic; Kathryn G Commons
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-10-13
Journal Detail:
Title:  Brain research     Volume:  1306     ISSN:  1872-6240     ISO Abbreviation:  Brain Res.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-11-27     Completed Date:  2010-02-03     Revised Date:  2014-09-13    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  29-38     Citation Subset:  IM    
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MeSH Terms
Analgesics, Opioid / pharmacology*
Brain Stem / drug effects*,  metabolism
Cell Count
Microscopy, Fluorescence
Morphine / pharmacology*
Neurons / drug effects,  metabolism
Norepinephrine / metabolism
Pain / chemically induced,  drug therapy*,  metabolism*
Proto-Oncogene Proteins c-fos / metabolism*
Rats, Sprague-Dawley
Serotonin / metabolism
Time Factors
Grant Support
DA-021801/DA/NIDA NIH HHS; R01 DA021801/DA/NIDA NIH HHS; R01 DA021801-02/DA/NIDA NIH HHS
Reg. No./Substance:
0/Analgesics, Opioid; 0/Proto-Oncogene Proteins c-fos; 1HG84L3525/Formaldehyde; 333DO1RDJY/Serotonin; 76I7G6D29C/Morphine; X4W3ENH1CV/Norepinephrine

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