| Visualizing light-triggered release of molecules inside living cells. | |
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MedLine Citation:
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PMID: 20857946 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The light-triggered release of deoxyribonucleic acid (DNA) from gold nanoparticle-based, plasmon resonant vectors, such as nanoshells, shows great promise for gene delivery in living cells. Here we show that intracellular light-triggered release can be performed on molecules that associate with the DNA in a DNA host-guest complex bound to nanoshells. DAPI (4',6-diamidino-2-phenylindole), a bright blue fluorescent molecule that binds reversibly to double-stranded DNA, was chosen to visualize this intracellular light-induced release process. Illumination of nanoshell-dsDNA-DAPI complexes at their plasmon resonance wavelength dehybridizes the DNA, releasing the DAPI molecules within living cells, where they diffuse to the nucleus and associate with the cell's endogenous DNA. The low laser power and irradiation times required for molecular release do not compromise cell viability. This highly controlled co-release of nonbiological molecules accompanying the oligonucleotides could have broad applications in the study of cellular processes and in the development of intracellular targeted therapies. |
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Authors:
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Ryan Huschka; Oara Neumann; Aoune Barhoumi; Naomi J Halas |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nano letters Volume: 10 ISSN: 1530-6992 ISO Abbreviation: Nano Lett. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-14 Completed Date: 2011-02-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088070 Medline TA: Nano Lett Country: United States |
Other Details:
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Languages: eng Pagination: 4117-22 Citation Subset: IM |
Affiliation:
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Department of Electrical and Computer Engineering, Rice University, 6100 Main Street, Houston, Texas 77005, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line, Tumor Cell Survival DNA / administration & dosage*, analysis* Delayed-Action Preparations / chemistry* Fluorescent Dyes / analysis Humans Indoles / analysis Light Nanoshells / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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P30CA16672/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Delayed-Action Preparations; 0/Fluorescent Dyes; 0/Indoles; 47165-04-8/DAPI; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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