| Visualizing ascorbate-triggered release of labile copper within living cells using a ratiometric fluorescent sensor. | |
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MedLine Citation:
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PMID: 20052977 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We present the synthesis, properties, and biological applications of Ratio-Coppersensor-1 (RCS1), a new water-soluble fluorescent sensor for ratiometric imaging of copper in living cells. RCS1 combines an asymmetric BODIPY reporter and thioether-based ligand receptor to provide high selectivity and sensitivity for Cu(+) over other biologically relevant metal ions, including Cu(2+) and Zn(2+), a ca. 20-fold fluorescence ratio change upon Cu(+) binding, and visible excitation and emission profiles compatible with standard fluorescence microscopy filter sets. Live-cell confocal microscopy experiments show that RCS1 is membrane-permeable and can sense changes in the levels of labile Cu(+) pools within living cells by ratiometric imaging, including expansion of endogenous stores of exchangeable intracellular Cu(+) triggered by ascorbate stimulation in kidney and brain cells. |
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Authors:
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Dylan W Domaille; Li Zeng; Christopher J Chang |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of the American Chemical Society Volume: 132 ISSN: 1520-5126 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-27 Completed Date: 2010-04-05 Revised Date: 2011-07-22 |
Medline Journal Info:
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Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
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Languages: eng Pagination: 1194-5 Citation Subset: IM |
Affiliation:
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Department of Chemistry and the Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Ascorbic Acid / chemistry* Brain / cytology Cell Line, Tumor Copper / analysis* Fluorescent Dyes / chemical synthesis* Kidney / cytology Microscopy, Confocal / methods* Molecular Structure Rats |
| Grant Support | |
ID/Acronym/Agency:
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GM 79465/GM/NIGMS NIH HHS; R01 GM079465-03/GM/NIGMS NIH HHS; R01 GM079465-04/GM/NIGMS NIH HHS; T32GM066698/GM/NIGMS NIH HHS; //Howard Hughes Medical Institute |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 50-81-7/Ascorbic Acid; 7440-50-8/Copper |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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