Document Detail


Visualization of transfection of hepatocytes by galactosylated chitosan-graft-poly(ethylene glycol)/DNA complexes by confocal laser scanning microscopy.
MedLine Citation:
PMID:  12711166     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dual-labeled galactosylated chitosan-graft-poly(ethylene glycol) (PEG) (GCP)/DNA complexes were prepared and their hepatocyte-specific delivery and cellular distribution were investigated by confocal laser scanning microscopy (CLSM). The complexes were transfected into hepatocyte through specific interaction of galactose moiety of the GCP and asialoglycoprotein receptors (ASGPR) of the hepatocytes. The GCP/DNA complexes taken up by the hepatocytes were rapidly released into the cytoplasm, but nuclear trafficking of the released complexes was slow and rate-limiting process. The more efficient transfection of the complex occurred in the human-derived HepG2 cells than in primary hepatocytes.
Authors:
I K Park; T H Kim; S I Kim; Y H Park; W J Kim; T Akaike; C S Cho
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of pharmaceutics     Volume:  257     ISSN:  0378-5173     ISO Abbreviation:  Int J Pharm     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-24     Completed Date:  2003-05-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7804127     Medline TA:  Int J Pharm     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  103-10     Citation Subset:  IM    
Affiliation:
School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Asialoglycoprotein Receptor / metabolism
Cell Line
Chitin / administration & dosage*,  analogs & derivatives*
Chitosan
DNA / administration & dosage*
Female
Galactose / metabolism*
Hepatocytes / metabolism*
Humans
Mice
Mice, Inbred ICR
Microscopy, Confocal
Particle Size
Polyethylene Glycols / administration & dosage*
Transfection*
Chemical
Reg. No./Substance:
0/Asialoglycoprotein Receptor; 0/Polyethylene Glycols; 1398-61-4/Chitin; 26566-61-0/Galactose; 9007-49-2/DNA; 9012-76-4/Chitosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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