| Visualization of transfection of hepatocytes by galactosylated chitosan-graft-poly(ethylene glycol)/DNA complexes by confocal laser scanning microscopy. | |
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MedLine Citation:
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PMID: 12711166 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dual-labeled galactosylated chitosan-graft-poly(ethylene glycol) (PEG) (GCP)/DNA complexes were prepared and their hepatocyte-specific delivery and cellular distribution were investigated by confocal laser scanning microscopy (CLSM). The complexes were transfected into hepatocyte through specific interaction of galactose moiety of the GCP and asialoglycoprotein receptors (ASGPR) of the hepatocytes. The GCP/DNA complexes taken up by the hepatocytes were rapidly released into the cytoplasm, but nuclear trafficking of the released complexes was slow and rate-limiting process. The more efficient transfection of the complex occurred in the human-derived HepG2 cells than in primary hepatocytes. |
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Authors:
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I K Park; T H Kim; S I Kim; Y H Park; W J Kim; T Akaike; C S Cho |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of pharmaceutics Volume: 257 ISSN: 0378-5173 ISO Abbreviation: Int J Pharm Publication Date: 2003 May |
Date Detail:
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Created Date: 2003-04-24 Completed Date: 2003-05-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7804127 Medline TA: Int J Pharm Country: Netherlands |
Other Details:
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Languages: eng Pagination: 103-10 Citation Subset: IM |
Affiliation:
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School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, South Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Asialoglycoprotein Receptor / metabolism Cell Line Chitin / administration & dosage*, analogs & derivatives* Chitosan DNA / administration & dosage* Female Galactose / metabolism* Hepatocytes / metabolism* Humans Mice Mice, Inbred ICR Microscopy, Confocal Particle Size Polyethylene Glycols / administration & dosage* Transfection* |
| Chemical | |
Reg. No./Substance:
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0/Asialoglycoprotein Receptor; 0/Polyethylene Glycols; 1398-61-4/Chitin; 26566-61-0/Galactose; 9007-49-2/DNA; 9012-76-4/Chitosan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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