| Visualization and quantification of whole rat heart laminar structure using high-spatial resolution contrast-enhanced MRI. | |
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MedLine Citation:
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PMID: 22021329 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It has been shown by histology that cardiac myocytes are organized into laminae and this structure is important in function, both influencing the spread of electrical activation and enabling myocardial thickening in systole by laminar sliding. We have carried out high-spatial resolution three-dimensional MRI of the ventricular myolaminae of the entire volume of the isolated rat heart after contrast perfusion [dimeglumine gadopentate (Gd-DTPA)]. Four ex vivo rat hearts were perfused with Gd-DTPA and fixative and high-spatial resolution MRI was performed on a 9.4T MRI system. After MRI, cryosectioning followed by histology was performed. Images from MRI and histology were aligned, described, and quantitatively compared. In the three-dimensional MR images we directly show the presence of laminae and demonstrate that these are highly branching and are absent from much of the subepicardium. We visualized these MRI volumes to demonstrate laminar architecture and quantitatively demonstrated that the structural features observed are similar to those imaged in histology. We showed qualitatively and quantitatively that laminar architecture is similar in the four hearts. MRI can be used to image the laminar architecture of ex vivo hearts in three dimensions, and the images produced are qualitatively and quantitatively comparable with histology. We have demonstrated in the rat that: 1) laminar architecture is consistent between hearts; 2) myolaminae are absent from much of the subepicardium; and 3) although localized orthotropy is present throughout the myocardium, tracked myolaminae are branching structures and do not have a discrete identity. |
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Authors:
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Stephen H Gilbert; David Benoist; Alan P Benson; Ed White; Steven F Tanner; Arun V Holden; Halina Dobrzynski; Olivier Bernus; Aleksandra Radjenovic |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Video-Audio Media Date: 2011-10-21 |
Journal Detail:
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Title: American journal of physiology. Heart and circulatory physiology Volume: 302 ISSN: 1522-1539 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2011-12-27 Completed Date: 2012-02-14 Revised Date: 2012-05-23 |
Medline Journal Info:
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Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States |
Other Details:
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Languages: eng Pagination: H287-98 Citation Subset: IM |
Affiliation:
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Institute of Membrane and Systems Biology, University of Leeds, United Kingdom. s.h.gilbert@leeds.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Contrast Media / diagnostic use* Fixatives Formaldehyde Gadolinium DTPA / diagnostic use* Heart / anatomy & histology* Image Interpretation, Computer-Assisted Imaging, Three-Dimensional Magnetic Resonance Imaging* Male Perfusion Rats Rats, Wistar Reproducibility of Results Tissue Fixation |
| Grant Support | |
ID/Acronym/Agency:
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088908/Z/09/Z//Wellcome Trust; G0701776//Medical Research Council; G0701785//Medical Research Council; G900524//Medical Research Council |
| Chemical | |
Reg. No./Substance:
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0/Contrast Media; 0/Fixatives; 50-00-0/Formaldehyde; 80529-93-7/Gadolinium DTPA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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