Document Detail


Visualization of mitotic chromosomes in filamentous fungi by fluorescence staining and fluorescence in situ hybridization.
MedLine Citation:
PMID:  7859561     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mitotic chromosomes of the plant pathogenic filamentous fungi Botrytis cinerea and Alternaria alternata were observed. Chromosomes prepared by the germ tube burst method were stained with the fluorescent dye 4',6-diamidino-2-phenylindole (DAPI) to yield figures with good resolution. Using this method, component chromosomes were clearly distinguished and the chromosome number could be determined. Fluorescence in situ hybridization (FISH) was also successfully applied to the specimens, revealing one ribosomal RNA gene cluster, or nucleolus organizer region (NOR) in the genome of each fungus. A long attenuated chromatid thread expanding from a condensed metaphase chromosome, which had been called a thread-like structure in B. cinerea, was proved to be an NOR. This is the first report of the successful application of FISH to the chromosomes of filamentous fungi.
Authors:
M Taga; M Murata
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chromosoma     Volume:  103     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1995-03-23     Completed Date:  1995-03-23     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  408-13     Citation Subset:  IM    
Affiliation:
College of General Education, Okayama University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alternaria / ultrastructure*
Chromosomes, Fungal*
DNA, Fungal / analysis
DNA, Ribosomal / analysis
Fluorescent Dyes
In Situ Hybridization, Fluorescence / methods*
Indoles
Karyotyping / methods
Mitosis
Mitosporic Fungi / ultrastructure*
Nucleolus Organizer Region / ultrastructure
Chemical
Reg. No./Substance:
0/DNA, Fungal; 0/DNA, Ribosomal; 0/Fluorescent Dyes; 0/Indoles; 47165-04-8/DAPI

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