Document Detail

Visualization of leukocyte dynamics in the choroid with indocyanine green.
MedLine Citation:
PMID:  8843909     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To report a method to evaluate leukocyte dynamics in the choroidal circulation with indocyanine green (ICG) angiography. METHODS: Nonpigmented and pigmented rats were administered ICG solution intravenously. The fundus image was obtained with a scanning laser ophthalmoscope and recorded on magnetic tapes at a video rate (30 frames/second). The images were analyzed with a personal computer-based image analysis system. RESULTS: On ICG angiography, hyperfluorescent dots were seen moving along the choroidal vessels and in the retinal vessels several minutes after injection. These fluorescent dots were thought to be circulating leukocytes stained with ICG. The micrographs of blood smears after ICG injection showed intense fluorescence of leukocytes. Computer-assisted image analysis allowed tracing of these fluorescent dots using a frame-by frame method. CONCLUSIONS: Results of this study indicated that ICG can be used for vital staining of leukocytes and that it is possible to evaluate leukocyte movement in the choroidal circulation in vivo in rats.
N Matsuda; Y Ogura; H Nishiwaki; K Miyamoto; T Matsubara; J Kiryu; Y Honda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  37     ISSN:  0146-0404     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1996-11-06     Completed Date:  1996-11-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2228-33     Citation Subset:  IM    
Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, Kyoto University, Japan.
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MeSH Terms
Arterioles / physiology
Blood Circulation
Chemotaxis, Leukocyte / physiology
Choroid / blood supply*
Fluorescein Angiography / methods*
Image Processing, Computer-Assisted
Indocyanine Green / diagnostic use*
Lasers / diagnostic use
Leukocytes / physiology*
Rats, Inbred WKY
Venules / physiology
Reg. No./Substance:
3599-32-4/Indocyanine Green

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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